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Establishment of a markerless multiple-gene deletion method based on Cre/loxP mutant system for Bacillus pumilus

文献类型： 外文期刊

作者： Guan, Zheng-Bing 1 ; Wang, Kai-Qiang 1 ; Shui, Yan 2 ; Liao, Xiang-Ru 1 ;

作者机构： 1.Jiangnan Univ, Key Lab Ind Biotechnol, Minist Educ, Sch Biotechnol, Wuxi 214122, Peoples R China

2.Chinese Acad Fishery Sci, Key Lab Freshwater Fisheries & Germplasm Resource, Minist Agr, Freshwater Fisheries Res Ctr, Wuxi, Peoples R China

关键词： Bacillus pumilus;Cre;loxP system;gene deletion;recombination

期刊名称：JOURNAL OF BASIC MICROBIOLOGY （ 影响因子：2.281； 五年影响因子：2.516 ）

ISSN： 0233-111X

年卷期： 2017 年 57 卷 12 期

页码：

收录情况： SCI

摘要： In this study, we established a Cre/loxP mutant recombination system (Cre/lox71-66 system) for markerless gene deletion to facilitate our follow-up rational genetic engineering to the strain Bacillus pumilus W3. This modified method uses two mutant loxP sites, which after recombination creates a double-mutant loxP site that is poorly recognized by Cre recombinase, facilitating multiple gene deletions in a single genetic background. Two selected genes, cotA and sigF, were continuously knocked out and verified at different levels using this method. This method is simple and efficient and can be easily implemented for multiple gene deletions in B. pumilus.

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