Transparent Tiger barb Puntius tetrazona, a fish model for in vivo analysis of nocardial infection
文献类型： 外文期刊
作者： Wang, F. 1 ; Wang, X. G. 2 ; Liv, C. 1 ; Chang, O. Q. 1 ; Feng, Y. Y. 1 ; Jiang, L. 1 ; Li, K. B. 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Pearl River Fishery Res Inst, Key Lab Aquat Anim Immune Technol Guangdong Prov, Guangzhou 510380, Guangdong, Peoples R China
2.Guangdong Med Univ, Clin Res Ctr, Zhanjiang 524001, Guangdong, Peoples R China
关键词： Puntius tetrazona;transparent;leukocyte;in vivo;Nocardia seriolae;granuloma
期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）
ISSN： 0378-1135
年卷期： 2017 年 211 卷
页码：
收录情况： SCI
摘要： Nocardiosis afflicts multiple species of cultured fish, resulting in substantial economic losses to the aquaculture industry, however, lack of detailed knowledge on disease pathogenesis has hampered the development of effective prevention and control strategies. In this study, we injected a green fluorescent protein (GFP)-labeled Nocardia seriolae strain into a transparent mutant strain of Tiger barb (Puntius tetrazona) to monitor tissue pathogen accumulation and tissue damage in vivo, and to clarify the relationship between pathogenic processes and overt symptoms. GFP-labeled bacteria were phagocytized by leukocytes and could proliferate within these cells, which in turn led to leukocyte aggregation, leukocyte death, and granuloma formation. In addition, intracellular bacteria could permanently colonize various tissues via leukocyte circulation, causing multi-organ infection as revealed by changes of tissue transparency. Histology revealed granulomatous lesions in organs such as muscle, kidney, and spleen that was corresponded to the tissue opacities in vivo. Confocal microscopy confirmed massive accumulations of GFP-labeled bacteria within these granulomas, which often contained a necrotic core. Tiger barb transparency allows for real-time observation of in vivo pathological changes within the same animal, and the pathogenic process can be evaluated based on the shape and size of body opacities. Thus, transparent Tiger barb is a promising model to study the pathogenesis of nocardiosis.
- 相关文献
作者其他论文 更多>>
-
The complete mitochondrial genome of the Brachymystax tsinlingensis Li
作者：Xiong, D. M.;Shao, J.;Jiang, H. B.;Luo, W.;Jiang, H. B.;Shao, J.;Wang, F.;Lu, B.
关键词：Brachymystax tsinlingensis Li; complete mitochondrial genome; phylogeny tree
-
Length-weight relationships for 13 fish species in the Tangwang River, a tributary of Songhua River, China
作者：Wang, J. L.;Liu, W.;Ying, Ch.;Wang, F.;Li, P. L.;Wang, Ch.;Tang, F. J.;Ying, Ch.;Wang, F.;Li, P. L.;Wang, Ch.
关键词：
-
Length-weight relationships of three fish species from the Tibet reach of the Lancang River, China
作者：Zhu, T. B.;Wang, X. G.;Huang, J.;Yang, D. G.
关键词：
-
Aeromonas shuberti as a cause of multi-organ necrosis in internal organs of Nile tilapia, Oreochromis niloticus
作者：Liu, C.;Chang, O. Q.;Zhang, D. F.;Li, K. B.;Wang, F.;Lin, M. H.;Shi, C. B.;Jiang, L.;Wang, Q.;Bergmann, S. M.
关键词：Aeromonas schubertii; infection; nile tilapia; Nodules; pathogenicity
-
Summer and winter living coccolithophores in the Yellow Sea and the East China Sea
作者：Sun, J.;Gu, X. Y.;Feng, Y. Y.;Jin, S. F.;Jiang, W. S.;Jin, H. Y.;Chen, J. F.
关键词：
-
Primary genome scan for complex body shape-related traits in the common carp Cyprinus carpio
作者：Zhang, Y.;Wang, S.;Li, J.;Jiang, L.;Xu, P.;Sun, X.;Zhang, X.;Lu, C.;Sun, X.;Lu, C.;Wan, Y.
关键词：body size traits;linkage mapping;quantitative trait locus
-
Ontogenetic Development of the Digestive Tract in Larvae of American Shad
作者：Hong, X. Y.;Zhu, X. P.;Chen, K. C.;Pan, D. B.;Li, K. B.;Zhu, X. P.
关键词：
