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Genetic Variability of Koi Herpesvirus In vitro-A Natural Event?

文献类型： 外文期刊

作者： Klafack, Sandro 1 ; Wang, Qing 2 ; Zeng, Weiwei 2 ; Wang, Yingying 2 ; Li, Yingying 2 ; Zheng, Shucheng 2 ; Kempter, J 1 ;

作者机构： 1.Friedrich Loeffler Inst, Fed Res Inst Anim Hlth, Greifswald, Germany

2.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou, Guangdong, Peoples R China

3.West Pomeranian Univ Technol, Dept Aquaculture, Szczecin, Poland

4.GeneReach Biotechnol Corp, Dept Res & Dev, Taichung, Taiwan

5.Natl Inst Vet Res, Pulawy, Poland

关键词： KHV;VNTR;Cyprinus carpio;molecular tracing;CyHV3

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2017 年 8 卷

页码：

收录情况： SCI

摘要： Worldwide koi herpesvirus (KHV) causes high mortalities in Cyprinus carpio L. aquaculture. So far, it is unknown how the different variants of KHV have developed and how they spread in the fish, but also in the environmental water bodies. Therefore, a phylogenetic method based on variable number of tandem repeats (VNTR) was improved to gain deeper insights into the phylogeny of KHV and its possible worldwide distribution. Moreover, a VNTR-3 qPCR was designed which allows fast virus typing. This study presents a useful method for molecular tracing of diverse KHV types, variants, and lineages.

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