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PdS Nanoparticle Label Based DNA Biosensor for Rapid Detection of Mercury (II)

文献类型： 外文期刊

作者： Wu, LiDong 1 ; Ding, YanHua 2 ; Hong, Nian 2 ; Cheng, Lin 2 ; Fan, Hao 2 ; Song, Yi 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Beijing 100141, Peoples R China

2.JiangXi Univ Tradit Chinese Med, Dept Pharm, Nanchang 330004, Jiangxi, Peoples R China

关键词： Electrochemical biosensor;Mercury (II);DNA hybridization;PdS nanoparticles;T-Tmismatches

期刊名称：INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE （ 影响因子：1.765； 五年影响因子：1.685 ）

ISSN： 1452-3981

年卷期： 2014 年 9 卷 12 期

页码：

收录情况： SCI

摘要： In the present study, we describe an electrochemical biosensor for Hg2+ detection by using the DNA probe. Two specific sequences for the probes, one has to ensure that the probe A hybridized with probe B modified on PdS nanoparticle to form stable DNA duplexes only in the presence of Hg2+ at a given operating temperature. After dissolving the PdS particles from the electrode, a mercury-film electrode is used for electrochemical detection of these Pd2+ ions which offer sensitively electrochemical signal transduction. The limit of detection of this assay in buffer is 16 nmol L-1. The detection is also specific for Hg2+ without being affected by the other metal ions, such as Cd2+, Li+, Ba2+, K+, Ca2+.

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