文献类型： 外文期刊

作者： Xu, Wei ¹ ; Kenez, Akos ² ; Mann, Sabine ³ ; Overton, Thomas R. ⁴ ; Wakshlag, Joseph J. ⁵ ; Nydam, Daryl, V ³ ; Feng, Tao ⁶ ; Yepes, Francisco Leal ⁷ ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Intelligent Equipment Res Ctr, Beijing 100097, Peoples R China

2.City Univ Hong Kong, Dept Infect Dis & Publ Hlth, Hong Kong, Peoples R China

3.Cornell Univ, Coll Vet Med, Dept Populat Med & Diagnost Sci, Ithaca, NY 14853 USA

4.Cornell Univ, Coll Vet Med, Dept Anim Sci, Ithaca, NY 14853 USA

5.Cornell Univ, Coll Vet Med, Dept Clin Sci, Ithaca, NY 14853 USA

6.Beijing Acad Agr & Forestry Sci, Inst Anim Husb & Vet Med, Beijing 100097, Peoples R China

7.Washington State Univ, Coll Vet Med, Dept Vet Clin Sci, Pullman, WA 99164 USA

8.Katholieke Univ Leuven, Dept Biosyst Biosyst Technol Cluster, Kleinhoefstr 4, B-2440 Geel, Belgium

关键词： branched-chain amino acid; metabolomics; phosphocholine; glutathione; milk metabolome

期刊名称：JOURNAL OF DAIRY SCIENCE （ 影响因子：4.225； 五年影响因子：4.987 ）

ISSN： 0022-0302

年卷期： 2022 年 105 卷 10 期

页码：

收录情况： SCI

摘要： The 3 branched-chain AA (BCAA), Val, Leu, and Ile, are essential AA used by tissues as substrates for protein synthesis and energy generation. In addition, BCAA are also involved in modulating cell signaling pathways, such as nutrient sensing and insulin signaling. In our previous study, dietary BCAA supplementation was shown to improve protein synthesis and glucose homeostasis in transition cows. However, a more detailed understanding of the changes in metabolic pathways associated with an increased BCAA availability is desired to fine-tune nutritional supplementation strategies. Multiparous Holstein cows (n = 20) were enrolled 28 d before expected calving and assigned to either the BCAA treatment (n = 10) or the control group (n = 10). Cows assigned to BCAA were fed 550 g/d of rumenprotected BCAA mixed with 200 g/d of dry molasses from calving until 35 DIM, whereas the cows assigned to the control were fed only 200 g/d of dry molasses. Serum samples were collected on d 10 before expected calving, as well as on d 4 and d 21 postpartum. Milk samples were collected on d 14 postpartum. From a larger cohort, we selected 20 BCAA-supplemented cows with the greatest plasma urea nitrogen concentration, as an indicator for greater BCAA availability, for the metabolomics analysis herein. Serum and milk samples were subjected to a liquid chromatography-mass spectrometry-based assay, detecting and measuring the abundance of 241 serum and 211 milk metabolic features, respectively. Multivariable statistical analyses revealed that BCAA supplementation altered the metabolome profiles of both serum and milk samples. Increased abundance of serum phosphocholine and glutathione and of milk Val, Ile, and Leu, and decreased abundance of milk acyl-carnitines were associated with BCAA supplementation. Altered phosphocholine and glutathione abundances point to altered hepatic choline metabolism and antioxidant balance, respectively. Altered milk acyl-carnitine abundances suggest changes in mammary fatty acid metabolism. Dietary BCAA supplementation was associated with a range of alterations in serum and milk metabolome profiles, adding to our understanding of the role of BCAA availability in modulating dairy cow protein, lipid, and energy metabolism on a whole-body level and how it affects milk composition.