您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

scFv antibodies against infectious bursal disease virus isolated from a combinatorial antibody library by flow cytometry

文献类型： 外文期刊

作者： Xu, Li-Ming 1 ; Li, Tian-He 2 ; Zhou, Bing 2 ; Guo, Mo 2 ; Liu, Miao 2 ; Zhao, Jing-Zhuang 2 ; Cao, Hong-Wei 3 ; Li, De-S 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Heilongjiang River Fishery Res Inst, Harbin 150070, Peoples R China

2.Northeast Agr Univ, Coll Life Sci, Harbin 150030, Peoples R China

3.HeiLongJiang BaYi Agr Univ, Coll Biol Sci & Technol, DaQing 163319, Peoples R China

关键词： Antibody library;Bacterial display library;Bursal disease virus;Infectious bursal disease virus;Spheroplasts;Viral proteins;VP2 protein

期刊名称：BIOTECHNOLOGY LETTERS （ 影响因子：2.461； 五年影响因子：2.457 ）

ISSN： 0141-5492

年卷期： 2014 年 36 卷 5 期

页码：

收录情况： SCI

摘要： Infectious bursal disease is an economically important disease that affects chickens worldwide. Here, a recombinant single chain variable fragment (scFv) antibody library derived from chickens immunized with VP2 protein of infectious bursal disease virus (IBDV) was constructed. The library was subjected to three rounds of screening by flow cytometry against VP2 protein through a bacteria display technology, resulting in the enrichment of scFv. Three scFv clones with different fluorescence intensity were obtained by random colony pick up. The isolated scFv antibodies were expressed and purified. Relative affinity assay showed the three clones had different sensitivity to VP2, in accordance with fluorescence activity cell sorting analysis. The potential use of the selected IBDV-specific scFv antibodies was demonstrated by the successful application of the isolated antibodies in western blotting assay and ELISA.

  • 相关文献

[1]High throughput screening of recombinant antibodies against infectious hematopoietic necrosis virus from a combinatorial antibody library. Xu, Li-Ming,Zhao, Jing-Zhuang,Liu, Miao,Cao, Yong-Sheng,Yin, Jia-Sheng,Liu, Hong-Bai,Tongyan-Lu.

[2]Anchored periplasmic expression (APEx)-based bacterial display for rapid and high-throughput screening of B cell epitopes. Guo, Mo,Zhou, Bing,Yin, Jie-Chao,Ye, Xian-Long,Ren, Gui-Ping,Li, De-Shan,Xu, Li-Ming.

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台