QTL for Short-Duration Vigorous Swimming Movements in Common Carp (Cyprinus carpio L.) Based on LDH Activity
文献类型： 外文期刊
作者： Punhal, Lashari 1 ; Laghari, Muhammad Younis 1 ; Narejo, Naeem Tariq 2 ; Zhang, Xiaofeng 1 ; Xu, Peng 1 ; Sun, Xiao 1 ;
作者机构： 1.Chinese Acad Fishery Sci, Ctr Appl Aquat Genom, Beijing, Peoples R China
2.Univ Sindh, Dept Fresh Water Biol & Fisheries, Jamshoro, Pakistan
关键词： LDH activity;QTL;vigorous swimming
期刊名称：PAKISTAN JOURNAL OF ZOOLOGY （ 影响因子：0.831； 五年影响因子：0.768 ）
ISSN： 0030-9923
年卷期： 2014 年 46 卷 2 期
页码：
收录情况： SCI
摘要： Lactate dehydrogenase (LDH) enzyme activities in muscle was significantly related with burst swimming speed and endurance swimming capacity in a variety of fish. Quantitative trait loci (QTLs) associated with LDH were analyzed based on F1 family crossed by Hebao and Heilongjiang carp. A total of seven QTLs (qLDH1, qLDH6, qLDH7, qLDH21 qLDH22, qLDH45a and qLDH45b) were detected on six linkage groups (LG1, LG6, LG7, LG21; LG22, LG45), and the explained variances of LDH activity by these QTLs was more than 36%. Confidence intervals ranged from 2.0 to 10 cM in the present study. To increase the intensity of QTL mapping, four new microsatellite markers (CAFS02457, CAFS02458, CAFS02460, and CAFS02461) were developed from common carp genomic fragments. Intensity of candidate QTL intervals was increased (by > 5 cM) in four of the seven QTLs. The p-value of qLDH7 was 0.0007 analyzed by an ANOVA and explained 42.0% of the variance suggesting this QTL maybe a major QTL.
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