Novel polymorphic microsatellite markers isolated from the pen shell Atrina pectinata (Mollusca: Bivalvia: Pinnidae)
文献类型: 外文期刊
作者: Wu, B. 1 ; Bai, L. J. 1 ; Yang, A. G. 1 ; Zhou, L. Q. 1 ; Liu, Z. H. 1 ;
作者机构: 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Minist Agr, Key Lab Sustainable Dev Marine Fisheries, Qingdao, Peoples R China
2.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai, Peoples R China
关键词: Atrina pectinata;Microsatellite loci;Polymorphic
期刊名称:GENETICS AND MOLECULAR RESEARCH ( 影响因子:0.764; 五年影响因子:0.912 )
ISSN: 1676-5680
年卷期: 2014 年 13 卷 4 期
页码:
收录情况: SCI
摘要: In this study, we isolated 21 novel polymorphic microsatellite DNA loci from the pen shell Atrina pectinata using magnetic-bead hybridization enrichment. The characteristics of these loci were tested using a population of 30 individuals collected from the Penglai coast, Shandong Province. The number of alleles ranged from 2 to 13, and polymorphism information content (PIC) varied from 0.1730 to 0.8954. Values for observed heterozygosity (H-O) and expected heterozygosity (H-E) ranged from 0.0714 to 0.9231 and from 0.1948 to 0.9237, respectively. Four loci deviated significantly from Hardy-Weinberg equilibrium. The newly developed microsatellite markers will be beneficial in assessing the genetic diversity, population structure and genetic conservation of A. pectinata, and in other relevant research.
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