中国水产科学研究院机构知识库

Lipopolysaccharide and beta-1,3-glucan binding protein in the hard clam (Meretrix meretrix): Molecular characterization and expression analysis

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文献类型：外文期刊

作者： Liu, S. X. ¹ ; Qi, Z. H. ² ; Zhang, J. J. ¹ ; He, C. B. ³ ; Gao, X. G. ³ ; Li, H. J. ¹ ;

作者机构： 1.Natl Marine Environm Monitoring Ctr, State Ocean Adm, Key Lab Ecol Environm Coastal Areas, Dalian, Peoples R China

2.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangzhou, Guangdong, Peoples R China

3.Liaoning Ocean & Fisheries Sci Res Inst, Key Lab Marine Fishery Mol Biol Liaoning Prov, Dalian, Peoples R China

关键词： Meretrix meretrix;Hard clam;Gene cloning;Lipopolysaccharide and beta-1,3-glucan binding protein;mRNA expression

期刊名称：GENETICS AND MOLECULAR RESEARCH （影响因子：0.764；五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2014 年 13 卷 3 期

页码：

收录情况： SCI

摘要： Pattern recognition molecules play an important role in innate immunity by recognizing conserved molecular patterns that are present on the surface of invading microorganisms. In this study, a lipopolysaccharide and beta-1,3-glucan binding protein (LGBP) gene was cloned from the hard clam Meretrix meretrix (designated as Mm-LGBP) by the expressed sequence tags and rapid amplification of cDNA ends method. The cDNA was 1827 bp in length, consisting of a 71-bp 5'-terminal untranslated region, a 62-bp 3'UTR, and a 1734-bp open reading frame encoding a 577-amino acid polypeptide with an estimated molecular mass of 60.7 kDa and a theoretical isoelectric point of 5.56. Characteristic potential polysaccharide binding, cell adhesion, and glucanase motifs were identified in the Mm-LGBP, indicating that Mm-LGBP should be a new member of the LGBP family. Quantitative real-time polymerase chain reaction was developed to detect the mRNA expression level of Mm-LGBP in 6 different tissues. Higher-level mRNA expression of Mm-LGBP was detected in the gill and digestive gland tissues. The upregulation of Mm-LGBP mRNA after Vibrio anguillarum challenge showed that Mm-LGBP play a pivotal role in antibacterial immunity.

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