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Bioenzyme-nanoenzyme-chromogen all-in-one test strip for convenient and sensitive detection of malathion in water environment

文献类型： 外文期刊

作者： Zhang, Hanwen 1 ; Jin, Jiabin 2 ; Wang, Xini 1 ; Han, Wei 3 ; Qin, Lei 5 ; Chen, Lin 4 ; Mao, Xiaoyan 5 ; Liu, Zhenjiang 3 ; Xiang, Xingwei 1 ;

作者机构： 1.Zhejiang Univ Technol, Coll Food Sci & Technol, Hangzhou 310014, Zhejiang, Peoples R China

2.Natl Narcot Lab Zhejiang Reg Ctr NNLZRC, Hangzhou 310021, Peoples R China

3.Jiangsu Univ, Coll Environm & Safety Engn, Zhenjiang 212013, Jiangsu, Peoples R China

4.Zhejiang Acad Agr Sci, Inst Sericultural & Tea, Hangzhou 310021, Zhejiang, Peoples R China

5.Zhejiang Univ Technol, Ctr Membrane Separat & Water Sci & Technol, State Key Lab Base Green Chem Synth Technol, Hangzhou 310014, Peoples R China

关键词： Alkaline phosphatase; Malathion; Test strip; Rapid testing

期刊名称：SCIENCE OF THE TOTAL ENVIRONMENT （ 影响因子：8.2； 五年影响因子：8.6 ）

ISSN： 0048-9697

年卷期： 2024 年 924 卷

页码：

收录情况： SCI

摘要： The presence of pesticide residues in aquatic environments poses a significant threat to both aquatic ecosystems and human health. The presence of these residues can result in significant harm to aquatic ecosystems and can negatively impact the health of aquatic organisms. Consequently, this issue requires urgent attention and effective measures to mitigate its impact. However, developing sensitive and rapid detection methods remains a challenge. In this study, an all-in-one test strip, which integrated bioenzymes, nanoenzymes, and a chromogen, was developed in combination with an enzyme labeling instrument for a highly sensitive and convenient sensing of malathion residues. The oxidase activity of heme chloride (Hemin) in the strip can catalyze the oxidation of H2O2 and 3,3 ',5,5 '-tetramethylbenzidine (TMB) to produce a blue-colored oxide. Simultaneously, the alkaline phosphatase (ALP) present in the strip can break down L-ascorbic acid-2-phosphate to produce ascorbic acid (AA). This AA then acts to reduce the oxidized form of TMB, turning it into a colorless substance and leading to the disappearance of its fluorescent signal. In the presence of a pesticide, the activity of ALP is inhibited and formation of AA is blocked, thereby preventing the reduction of oxidized TMB and producing a colored signal. According to this principle, the integrated test strip detected the target pesticide with high performance as per the optical density value determined via an enzyme marker. The detection limit of the test strip was 0.209 ng/mL with good sensitivity. The method was used for detecting malathion in actual river water samples, and the recoveries were in the range of 93.53 %-96.87 %. The newly devised technique effectively identified malathion in samples of natural water. This research has introduced a novel approach for the precise and convenient surveillance of pesticide remnants. Additionally, these discoveries could inspire the advancement of proficient multi-enzyme detection systems.

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