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Lipid oxidation driven olefinic aldehyde biosynthesis shapes aged aroma in Qingzhuan tea

文献类型: 外文期刊

作者: Zheng, Pengcheng 1 ; Feng, Lin 1 ; Gao, Shiwei 1 ; Xue, Jinjin 1 ; Wang, Shengpeng 1 ; Wang, Xueping 1 ; Ye, Fei 1 ; Gui, Anhui 1 ; Teng, Jing 1 ; Luo, Rui 1 ; Chen, Jia 1 ; Liu, Zhonghua 2 ; Liu, Panpan 1 ;

作者机构: 1.Hubei Acad Agr Sci, Key Lab Tea Resources Comprehens Utilizat, Hubei Qingzhuan Tea Engn Res Ctr, Fruit & Tea Res Inst,Minist Agr & Rural Affairs, Wuhan 430064, Hubei, Peoples R China

2.Hunan Agr Univ, Key Lab Tea Sci, Minist Educ, Changsha 410128, Hunan, Peoples R China

3.Huazhong Agr Univ, Coll Hort & Forestry Sci, Wuhan 430070, Hubei, Peoples R China

关键词: Qingzhuan tea; Lipid metabolism; Unsaturated fatty acid; Olefinic aldehyde; Volatile compound; Qingzhuan tea; Lipid metabolism; Unsaturated fatty acid; Olefinic aldehyde; Volatile compound

期刊名称:FOOD CHEMISTRY-X ( 影响因子:8.2; 五年影响因子:8.2 )

ISSN: 2590-1575

年卷期: 2025 年 30 卷

页码:

收录情况: SCI

摘要: This study systematically investigates lipid dynamics and their role in aroma formation during Qingzhuan tea (QZT) processing. Using UHPLC-MRM-MS/MS and GC-MS, we analyzed fatty acids (FAs) and oxidized fatty acids (OFAs) across seven processing stages, identifying 31 FAs and 55 OFAs. Polyunsaturated fatty acids (PUFAs), particularly alpha-linolenic acid (C18:3) and linoleic acid (C18:2), dominated the lipid profiles (43.7 %-60.1 %), exhibiting biphasic dynamics: a 5.3-fold increase during pile fermentation and natural aging (RT -> A12) followed by oxidative degradation (30.0 % reduction in QZT). Multivariate analysis revealed 76 differential lipids correlating with 22 key volatiles, including (E,E)-2,4-heptadienal and (E)-2-octenal. Metabolic pathway analysis mapped lipoxygenase/cyclooxygenase (LOX/COX)-mediated oxidation of C18:3/C18:2 to hydroperoxides, which were then cleaved by lyases into aldehydes. Isotope labeling confirmed cross-pathway interactions between linoleic and arachidonic acid metabolism, while modeling experiments validated enzymatic generation of C6-C9 aldehydes from lipid precursors. This work elucidates the biochemical basis of QZT's aged aroma, providing actionable insights for flavor modulation in fermented teas.

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