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Comparative genomics of the medicinal plants Lonicera macranthoides and L. japonica provides insight into genus genome evolution and hederagenin-based saponin biosynthesis

文献类型： 外文期刊

作者： Yin, Xiaojian 1 ; Xiang, Yaping 1 ; Huang, Feng-Qing 1 ; Chen, Yahui 1 ; Ding, Hengwu 3 ; Du, Jinfa 1 ; Chen, Xiaojie 1 ; Wang, Xiaoxiao 1 ; Wei, Xinru 1 ; Cai, Yuan-Yuan 1 ; Gao, Wen 1 ; Guo, Dongshu 4 ; Alolga, Raphael N. N. 1 ; Kan, Xianzhao 3 ; Zhang, Baolong 4 ; Alejo-Jacuinde, Gerardo 5 ; Li, Ping 1 ; Tran, Lam-Son Phan 5 ; Herrera-Estrella, Luis 5 ; Lu, Xu 1 ; Qi, Lian-Wen 1 ;

作者机构： 1.China Pharmaceut Univ, Clin Metabol Ctr, Sch Tradit Chinese Pharm, Nanjing, Peoples R China

2.Chinese Acad Sci, Northeast Inst Geog & Agroecol, Key Lab Soybean Mol Design Breeding, Changchun, Peoples R China

3.Anhui Normal Univ, Inst Bioinformat, Coll Life Sci, Wuhu, Peoples R China

4.Jiangsu Acad Agr Sci, Prov Key Lab Agrobiol, Nanjing, Peoples R China

5.Texas Tech Univ, Inst Genom Crop Abiot Stress Tolerance, Dept Plant & Soil Sci, Lubbock, TX 79409 USA

6.Ctr Invest & Estudios Avanzados IPN, Lab Nacl Genomica, Unidad Genom Avanzada, Irapuato, Mexico

关键词： Caprifoliaceae (honeysuckle); chromosome-level genome; hederagenin-based saponins; Lonicere macranthoides; oleanolic acid synthase

期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：13.8； 五年影响因子：13.2 ）

ISSN： 1467-7644

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Lonicera macranthoides (LM) and L. japonica (LJ) are medicinal plants widely used in treating viral diseases, such as COVID-19. Although the two species are morphologically similar, their secondary metabolite profiles are significantly different. Here, metabolomics analysis showed that LM contained similar to 86.01 mg/g hederagenin-based saponins, 2000-fold higher than LJ. To gain molecular insights into its secondary metabolite production, a chromosome-level genome of LM was constructed, comprising 9 pseudo-chromosomes with 40 097 protein-encoding genes. Genome evolution analysis showed that LM and LJ were diverged 1.30-2.27 million years ago (MYA). The two plant species experienced a common whole-genome duplication event that occurred similar to 53.9-55.2 MYA before speciation. Genes involved in hederagenin-based saponin biosynthesis were arranged in clusters on the chromosomes of LM and they were more highly expressed in LM than in LJ. Among them, oleanolic acid synthase (OAS) and UDPglycosyltransferase 73 (UGT73) families were much more highly expressed in LM than in LJ. Specifically, LmOAS1 was identified to effectively catalyse the C-28 oxidation of b-Amyrin to form oleanolic acid, the precursor of hederagenin-based saponin. LmUGT73P1 was identified to catalyse cauloside A to produce a-hederin. We further identified the key amino acid residues of LmOAS1 and LmUGT73P1 for their enzymatic activities. Additionally, comparing with collinear genes in LJ, LmOAS1 and LmUGT73P1 had an interesting phenomenon of 'neighbourhood replication' in LM genome. Collectively, the genomic resource and candidate genes reported here set the foundation to fully reveal the genome evolution of the Lonicera genus and hederagenin-based saponin biosynthetic pathway.

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