您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Molecular cloning and functional characterization of cyclin E and CDK2 from Penaeus monodon

文献类型： 外文期刊

作者： Zhao, C. 1 ; Fu, M. J. 1 ; Qiu, L. H. 1 ;

作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangzhou, Guangdong, Peoples R China

2.Minist Agr, Key Lab South China Sea Fishery Resources Exploit, Guangzhou, Guangdong, Peoples R China

3.South China Sea Fisheries Res Inst, Trop Aquaculture Res & Dev Ctr, Sanya, Peoples R China

关键词： Cell cycle;Cyclin E/CDK2 complex;Prokaryotic expression;Pull-down;Penaeus monodon

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2016 年 15 卷 3 期

页码：

收录情况： SCI

摘要： Reduced reproductive performance of the black tiger shrimp (Penaeus monodon) has caused economic losses and hampered the fishing industry. Detailed investigation of the molecular mechanism by which the cell cycle is regulated in this organism is needed to understand the development and maturation of ovaries and oocytes, with a view to improving reproductive capacity. Cell cycle progression is mainly determined by cyclin-dependent kinase (CDK) and cyclin complexes, the cyclin E/CDK2 complex playing a key role in G1/S transition. However, knowledge of the interplay between cyclin E and CDK2 in invertebrates remains limited. In this study, full-length P. monodon cyclin E (Pmcyclin E) and CDK2 (PmCDK2) sequences were cloned. The open reading frame of Pmcyclin E was 1263 bp in length and encoded a 47.9-kDa protein, while that of PmCDK2 was 921 bp, encoding a protein of 34.9 kDa. Recombinant cyclin E and CDK2 proteins were expressed in Escherichia coli and purified by Ni-chelating affinity chromatography. In addition, a pull-down assay was performed to identify any interaction between Pmcyclin E and PmCDK2. This research provides a basis for the study of the functional mechanisms of the cyclin E/CDK2 complex in shrimp, further enriching our knowledge of invertebrate cell cycle regulation.

  • 相关文献

[1]南海西北部水域斑节对虾资源的调查研究. 钟振如,李辉权,张月平,陈敏. 1999

[2]The anaphase-promoting complex initiates zygote division in Arabidopsis through degradation of cyclin B1. Guo, Lei,Jiang, Li,Zhang, Ying,Lu, Xiu-Li,Liu, Chun-Ming,Guo, Lei,Xie, Qi,Weijers, Dolf,Jiang, Li.

[3]Identification and expression analysis of three c-type lysozymes in Oreochromis aureus. Gao, Feng-ying,Qu, Lan,Ye, Xing,Tian, Yuan-yuan,Zhang, Li-li,Bai, Jun-jie,Lu, Maixin,Qu, Lan,Ye, Xing,Yu, Shao-guo. 2012

[4]Cloning and prokaryotic expression of Chinese sturgeons cystatin cDNA. Bai, J.,Ma, D.,Lao, H.,Jian, Q.,Ye, X.,Luo, J.. 2005

[5]Identification and expression analysis of the g-type and c-type lysozymes in grass carp Ctenopharyngodon idellus. Ye, Xing,Zhang, Lili,Tian, Yuanyuan,Tan, Aiping,Bai, Junjie,Li, Shengjie,Zhang, Lili.

[6]Effect of guava leaves on growth and the non-specific immune response of Penaeus monodon. Yin, Xiao-Li,Li, Zhuo-Jia,Yang, Keng,Lin, Hei-Zhao,Guo, Zhi-Xun,Yin, Xiao-Li.

[7]Gene characteristics, immune and stress responses of PmPrx1 in black tiger shrimp (Penaeus monodon): Insights from exposure to pathogenic bacteria and toxic environmental stressors. Bu, Ruiqian,Wang, Pengfei,Zhao, Chao,Qiu, Lihua,Bu, Ruiqian,Bu, Ruiqian,Wang, Pengfei,Zhao, Chao,Qiu, Lihua,Qiu, Lihua,Bao, Weiyang.

[8]Characterization, expression and silencing by RNAi of p53 from Penaeus monodon. Dai, Wenting,Qiu, Lihua,Zhao, Chao,Fu, Mingjun,Ma, Zhenhua,Zhou, Falin,Yang, Qibin,Qiu, Lihua,Zhao, Chao,Fu, Mingjun,Ma, Zhenhua,Zhou, Falin,Yang, Qibin,Dai, Wenting,Yang, Qibin.

[9]Effect of seven carbohydrate sources on juvenile Penaeus monodon growth performance, nutrient utilization efficiency and hepatopancreas enzyme activities of 6-phosphogluconate dehydrogenase, hexokinase and amylase. Niu, Jin,Lin, Hei-Zhao,Jiang, Shi-Gui,Chen, Xu,Wu, Kai-Chang,Tian, Li-Xia,Liu, Yong-Jian.

[10]Molecular cloning, characterization and expression analysis of thrombospondin gene from Penaeus monodon. Zhou, FaLin,Zheng, Liming,Zhang, Dianchang,Huang, JianHua,Qiu, Lihua,Yang, QiBin,Jiang, ShiGui,Zhou, FaLin,Zheng, Liming.

[11]Estimates of heritability and genetic correlations for growth-related traits in the tiger prawn Penaeus monodon. Sun, Miao Miao,Huang, Jian Hua,Jiang, Shi Gui,Yang, Qi Bin,Zhou, Fa Lin,Zhu, Cai Yan,Yang, Li Shi,Su, Tian Feng.

[12]Cloning, characterization, expression analysis and RNAi of Retinoblastoma-like gene from black tiger shrimp (Penaeus monodon). Xie, Bobo,Wang, Pengfei,Zhao, Chao,Qiu, Lihua,Xie, Bobo,Xie, Bobo,Wang, Pengfei,Zhao, Chao,Qiu, Lihua,Qiu, Lihua.

[13]Molecular cloning and mRNA expression of M-phase phosphoprotein 6 gene in black tiger shrimp (Penaeus monodon). Zhou, Jun,Qiu, Lihua,Jiang, Shigui,Zhou, Falin,Huang, Jianhua,Yang, Lishi,Su, Tianfeng,Zhang, Dianchang,Zhou, Jun,Qiu, Lihua,Jiang, Shigui,Zhou, Falin,Huang, Jianhua,Yang, Lishi,Su, Tianfeng,Zhang, Dianchang.

[14]Observations of reproductive development and maturation of male Penaeus monodon reared in tidal and earthen ponds. Jiang, Shi-Gui,Huang, Jian-Hua,Zhou, Fa-Lin,Chen, Xu,Yang, Qj-Bing,Wen, Wei-Geng,Ma, Zi-Ming.

[15]Characterization of complement 1q binding protein of tiger shrimp, Penaeus monodon, and its C1q binding activity. Yang, Lishi,Liu, Xianjun,Liu, Wenjing,Li, Xiaolan,Qiu, Lihua,Huang, Jianhua,Jiang, Shigui,Liu, Xianjun,Liu, Wenjing,Li, Xiaolan. 2013

[16]Characterization of Argonaute2 gene from black tiger shrimp (Penaeus monodon) and its responses to immune challenges. Yang, Lishi,Li, Xiaolan,Jiang, Song,Qiu, Lihua,Zhou, Falin,Liu, Wenjing,Jiang, Shigui,Li, Xiaolan. 2014

[17]The response of glutathione peroxidase 1 and glutathione peroxidase 7 under different oxidative stresses in black tiger shrimp, Penaeus monodon. Liu, Wei,Zhao, Chao,Wang, Pengfei,Qiu, Lihua,Liu, Wei,Lin, Heizhao,Qiu, Lihua,Wang, Shu. 2018

[18]Morphometric analysis of four different populations of Penaeus monodon (Crustacea, Decapoda, Penaeidae). Sun, Miao Miao,Huang, Jian Hua,Jiang, Shi Gui,Yang, Qi Bin,Zhou, Fa Lin,Zhu, Cai Yan,Yang, Li Shi,Su, Tian Feng. 2013

[19]Characterization and expression analysis of a cyclin B gene from black tiger shrimp (Penaeus monodon). Zhao, C.,Fu, M. J.,Zhou, F. L.,Yang, Q. B.,Jiang, S. G.,Qiu, L. H.,Zhao, C.,Fu, M. J.,Zhou, F. L.,Yang, Q. B.,Jiang, S. G.,Qiu, L. H.,Yang, Q. B.. 2015

[20]Dietary values of astaxanthin and canthaxanthin in Penaeus monodon in the presence and absence of cholesterol supplementation: effect on growth, nutrient digestibility and tissue carotenoid composition. Niu, Jin,Li, Chun-Hou,Chen, Xu,Huang, Zhong,Lin, Hei-Zhao,Liu, Yong-Jian,Tian, Li-Xia. 2012

作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台