您好，欢迎访问贵州省农业科学院 机构知识库！
贵州省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

In vitro xanthine oxidase inhibitory properties of Flos Sophorae Immaturus and potential mechanisms

文献类型： 外文期刊

作者： Li, Jun 1 ; Gong, Yuhong 1 ; Li, Jinwei 1 ; Fan, Liuping 1 ;

作者机构： 1.Jiangnan Univ, State Key Lab Food Sci & Technol, 1800 Lihu Ave, Wuxi 214122, Jiangsu, Peoples R China

2.Jiangnan Univ, Sch Food Sci & Technol, 1800 Lihu Ave, Wuxi 214122, Jiangsu, Peoples R China

3.Jiangnan Univ, Collaborat Innovat Ctr Food Safety & Qual Control, 1800 Lihu Ave, Wuxi 214122, Jiangsu, Peoples R China

4.Guizhou Acad Agr Sci, Inst Food Proc Technol, Guiyang 550006, Peoples R China

关键词： Polyphenols; Flos sophorae immaturus; Xanthine oxidase; Interaction analysis; Inhibition stability; Fluorescence quenching

期刊名称：FOOD BIOSCIENCE （ 影响因子：5.318； 五年影响因子：5.846 ）

ISSN： 2212-4292

年卷期： 2022 年 47 卷

页码：

收录情况： SCI

摘要： Flos Sophorae Immaturus (FSI) possessed potential xanthine oxidase (XO) inhibitory activity as a uric acid-lowing natural product. The present work identified and quantified the free and bound polyphenols of FSI by UPLC-QTOF-MS. Then determined the primary polyphenols with XO inhibitory effect and clarified their potential mechanisms by omission experiment, interaction assay, inhibition type, and fluorescence measurements. The results revealed that nine polyphenols were detected in the free polyphenol extract and ten polyphenols were detected in the bound polyphenol extract. Meanwhile, seven polyphenols were identified as XO inhibitors, including quercetin, kaempferol, isorhamnetin, rutin, hyperoside, protocatechuic acid, and quercitrin with the IC50 values of 0.03, 0.11, 0.07, 5.62, 11.48, 22.13, and 367.82 mg/mL, but their inhibition stability was lower than 24 h. Although the content of quercetin (18.87 mg/g) was not the highest, it played a crucial role to the XO inhibitory effect of FSI. Furthermore, kaempferol and isorhamnetin alone revealed the sub-additive effect with quercetin, while the combination of other polyphenols with quercetin generated the interference or antagonism effects. Quercetin, isorhamnetin, and kaempferol were mixed-type and competitive inhibitors, which significantly quenched the fluorescence intensity of XO. Moreover, the binding processes of quercetin-XO, kaempferol-XO, and isorhamnetin-XO were spontaneous and endothermic, and the hydrophobic interaction was the key driving force. In general, quercetin, kaempferol, and isorhamnetin in FSI can be used as potential XO inhibitors.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：贵州省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台