文献类型： 外文期刊

作者： Yuan, Baoming ¹ ; Li, Beibei ¹ ; Shen, Hongfei ¹ ; Duan, Jiaqi ¹ ; Jia, Fenglian ¹ ; Maimaiti, Yushanjiang ⁴ ; Li, Yaning ² ; Li, Guangyue ¹ ;

作者机构： 1.Chinese Acad Agr Sci, Inst Plant Protect, Minist Agr, State Key Lab Biol Plant Dis & Insect Pests,Key La, Beijing 100081, Peoples R China

2.Hebei Agr Univ, Technol Innovat Ctr Biol Control Crop Dis & Insect, Coll Plant Protect, Baoding 071000, Peoples R China

3.Univ Liege, Funct & Evolutionary Entomol, Gembloux Agrobiotech, B-5030 Gembloux, Belgium

4.Xinjiang Acad Agr Sci, Key Lab Integrated Pest Management Crops Northwest, Minist Agr & Rural Affairs, Res Inst Plant Protect, Urumqi 83009, Xinjiang, Peoples R China

关键词： Rhizoctonia solani; Xenorhabdus budapestensis; fabclavine; biofungicide

期刊名称：JOURNAL OF APPLIED MICROBIOLOGY （ 影响因子：4.0； 五年影响因子：4.3 ）

ISSN： 1364-5072

年卷期： 2023 年 134 卷 9 期

页码：

收录情况： SCI

摘要： Aims Black scurf disease, caused by Rhizoctonia solani, is a severe soil-borne and tuber-borne disease, which occurs and spreads in potato growing areas worldwide and poses a serious threat to potato production. New biofungicide is highly desirable for addressing the issue, and natural products (NPs) from Xenorhabdus spp. provide prolific resources for biofungicide development. In this study, we aim to identify antifungal NPs from Xenorhabdus spp. for the management of this disease.Methods and results Out of the 22 Xenorhabdus strains investigated, Xenorhabdus budapestensis 8 (XBD8) was determined to be the most promising candidate with the measured IC50 value of its cell-free supernatant against R. solani as low as 0.19 ml l-1. The major antifungal compound in XBD8 started to be synthesized in the middle logarithmic phase and reached a stable level at stationary phase. Core gene deletion coupled with high-resolution mass spectrometry analysis determined the major antifungal NPs as fabclavine derivatives, Fcl-7 and 8, which showed broad-spectrum bioactivity against important pathogenic fungi. Impressively, the identified fabclavine derivatives effectively controlled black scurf disease in both greenhouse and field experiments, significantly improving tuber quality and increasing with marketable tuber yield from 29 300 to 35 494 kg ha-1, comparable with chemical fungicide fludioxonil.Conclusions The fabclavine derivatives Fcl-7 and 8 were determined as the major antifungal NPs in XBD8, which demonstrated a bright prospect for the management of black scurf disease.