文献类型： 外文期刊

作者： Zhang, Kekun ¹ ; Du, Mengrui ² ; Zhang, Hongyan ¹ ; Zhang, Xiaoqian ¹ ; Cao, Shuo ² ; Wang, Xu ² ; Wang, Wenrui ¹ ; Guan, Xueqiang ⁴ ; Zhou, Penghui ⁵ ; Li, Jin ⁵ ; Jiang, Wenguang ⁶ ; Tang, Meiling ⁷ ; Zheng, Qiuling ⁷ ; Cao, Muming ⁸ ; Zhou, Yongfeng ² ; Chen, Keqin ¹ ; Liu, Zhongjie ² ; Fang, Yulin ¹ ;

作者机构： 1.Northwest A&F Univ, Coll Enol, Heyang Viti Viniculture Stn, Ningxia Helan Mt East Foothill Wine Expt & Demonst, Yangling 712100, Peoples R China

2.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Minist Agr & Rural Affairs, Natl Key Lab Trop Crop Breeding,Shenzhen Branch,Gu, Shenzhen 518120, Peoples R China

3.Shanxi Agr Univ, Coll Agr, Taigu 030801, Peoples R China

4.Shandong Grape Res Inst, Shanda South Rd, Jinan 250199, Peoples R China

5.COFCO Great Wall Wine Penglai Co Ltd, Shandong Technol Innovat Ctr Wine Grape & Wine, Yantai 265600, Peoples R China

6.Yantai Changyu Grp Co Ltd, Yantai 264001, Peoples R China

7.Yantai Acad Agr Sci, Gangcheng West St, Yantai 264000, Peoples R China

8.Guangxi Acad Agr Sci, Viticulture & Wine Res Inst, Nanning 530007, Peoples R China

9.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Natl Key Lab Trop Crop Breeding, Haikou 570100, Peoples R China

期刊名称：HORTICULTURE RESEARCH （ 影响因子：8.7； 五年影响因子：9.0 ）

ISSN： 2662-6810

年卷期： 2023 年 10 卷 11 期

页码：

收录情况： SCI

摘要： Teinturier grapes are characterized by the typical accumulation of anthocyanins in grape skin, flesh, and vegetative tissues, endowing them with high utility value in red wine blending and nutrient-enriched foods developing. However, due to the lack of genome information, the mechanism involved in regulating teinturier grape coloring has not yet been elucidated and their genetic utilization research is still insufficient. Here, the cultivar 'Yan73' was used for assembling the telomere-to-telomere (T2T) genome of teinturier grapes by combining the High Fidelity (HiFi)? Hi-C and ultralong Oxford Nanopore Technologies (ONT) reads. Two haplotype genomes were assembled, at the sizes of 501.68 Mb and 493.38 Mb, respectively. In the haplotype 1 genome, the transposable elements (TEs) contained 32.77% of long terminal repeats (LTRs), while in the haplotype 2 genome, 31.53% of LTRs were detected in TEs. Furthermore, obvious inversions were identified in chromosome 18 between the two haplotypes. Transcriptome profiling suggested that the gene expression patterns in 'Cabernet Sauvignon' and 'Yan73' were diverse depending on tissues, developmental stages, and varieties. The transcription program of genes in the anthocyanins biosynthesis pathway between the two cultivars exhibited high similarity in different tissues and developmental stages, whereas the expression levels of numerous genes showed significant differences. Compared with other genes, the expression levels of VvMYBA1 and VvUFGT4 in all samples, VvCHS2 except in young shoots and VvPAL9 except in the E-L23 stage of 'Yan73' were higher than those of 'Cabernet Sauvignon'. Further sequence alignments revealed potential variant gene loci and structure variations of anthocyanins biosynthesis related genes and a 816 bp sequence insertion was found in the promoter of VvMYBA1 of 'Yan73' haplotype 2 genome. The 'Yan73' T2T genome assembly and comparative analysis provided valuable foundations for further revealing the coloring mechanism of teinturier grapes and the genetic improvement of grape coloring traits.