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AaTAS1 and AaMFS1 Genes for Biosynthesis or Efflux Transport of Tenuazonic Acid and Pathogenicity of Alternaria alternata

文献类型： 外文期刊

作者： Sun, Fan 1 ; Cao, Xueqiang 1 ; Yu, Dianzhen 1 ; Hu, Dongqiang 1 ; Yan, Zheng 1 ; Fan, Yingying 2 ; Wang, Cheng 2 ; Wu, Aibo 1 ;

作者机构： 1.Chinese Acad Sci, Univ Chinese Acad Sci, Shanghai Inst Nutr & Hlth, SIBS UGENT SJTU Joint Lab Mycotoxin Res,CAS Key L, Shanghai, Peoples R China

2.Xinjiang Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Key Lab Agroprod Qual & Safety Xinjiang,Minist Ag, Lab Qual & Safety Risk Assessment AgroProd Urumqi, Urumqi, Peoples R China

关键词： AaMFS1; AaTAS1; biosynthesis; efflux transport; tenuazonic acid

期刊名称：MOLECULAR PLANT-MICROBE INTERACTIONS （ 影响因子：3.422； 五年影响因子：4.274 ）

ISSN： 0894-0282

年卷期： 2022 年 35 卷 5 期

页码：

收录情况： SCI

摘要： Taking tenuazonic acid (TeA) synthetase 1 (TAS1) in Pyricularia oryzae as a reference, the homolog AaTAS1 was first anchored in Alternaria alternata via de novo sequencing. Subsequently, AaMFS1, as a major facilitator superfamily (MFS) protein-encoding gene in the adjacent upstream region, was followed with interest. As hypothesized, AaTAS1 is required for TeA biosynthesis, while AaMFS1 is an efflux pump for the transmembrane transport of TeA. Comparatively, the TeA yield of Delta AaTAS1 and Delta AaMFS1 dropped significantly compared with that of the wild-type strain. Specifically, the A domain of AaTAS1 catalyzed the start of TeA biosynthesis in vitro. Simultaneously, the pathogenicity of Delta AaTAS1 was also significantly decreased. Transcriptome analysis confirmed the abovementioned consistency between the TeA-producing phenotypes and related gene expression. Moreover, the proteins AaTAS1 and AaMFS1 were found present in the cytoplasm, plasma membrane, and intracellular membrane system, respectively, by fluorescence localization. Namely, AaTAS1 was responsible for the biosynthesis of TeA, and AaMFS1 was responsible for the efflux transport of TeA. Certainly, AaTAS1 indirectly regulated the expression of AaMFS1 through the level of synthetic TeA. Overall, data on the novel AaTAS1 and AaMFS1 genes mainly contribute to theoretical advances in mycotoxin biosynthesis and the pathogenicity of phytopathogens to agricultural foods.

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