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Identification and functional analysis of wheat lincRNAs in response to Fusarium graminearum infection

文献类型： 外文期刊

作者： Su, Chang 1 ; Li, Xue 1 ; Dong, Ye 1 ; Daniel, Bimpong 1 ; Liu, Chao 1 ; Xing, Yujun 2 ; Ma, Dongfang 1 ;

作者机构： 1.Yangtze Univ, Coll Agr, Key Lab Sustainable Crop Prod Middle Reaches Yangt, Minist Agr & Rural Affairs MARA, Jingzhou 434025, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, Jiangsu Key Lab Food Qual & Safety, Nanjing 210014, Peoples R China

3.Jingzhou Inst Technol, Coll Biochem Engn, Jingzhou 434020, Peoples R China

关键词： Fusarium graminearum; Intergenic long non-coding RNA; Virus-induced gene silencing; Molecular docking; Wheat (Triticum aestivum L.)

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：5.7； 五年影响因子：6.4 ）

ISSN： 0981-9428

年卷期： 2025 年 224 卷

页码：

收录情况： SCI

摘要： Intergenic long non-coding RNAs (lincRNAs) have recently been recognized as pivotal regulators in plant-pathogen interactions. However, the specific regulatory mechanisms of lincRNAs responding to Fusarium graminearum (F. graminearum) infection remain largely unexplored. Here, we performed time-series transcriptome profiling (0, 24, 48, and 72 h post-inoculation) and systematic identification of lincRNAs. A total of 1238 expressed lincRNAs were identified, among which 548 were differentially expressed lincRNAs during the time course of F. graminearum infection. We further predicted cis-regulatory lincRNA-mRNA pairs, comprising 347 lincRNAs and potential 1015 target genes, which were found to be mainly involved in amino acid metabolism and biosynthetic pathways. Moreover, 19 lincRNAs were predicted as putative precursors or endogenous target mimics of miRNAs. Subsequently, we verified that two lincRNAs, MSTRG.6494 and MSTRG.32080, showed strong transcriptional responses to F. graminearum infection by quantitative real-time PCR (qPCR) screening. Silencing MSTRG.6494 reduced the expression level of defense-related genes, resulting in reduced resistance to fungal pathogenicity. Meanwhile, the expression level of the potential target gene ATP synthase subunit beta (TaATP2) was significantly decreased in MSTRG.6494-silenced plants infected with F. graminearum. Overall, we performed the genome-wide identification of lincRNAs and their possible regulatory networks during F. graminearum infection-related process, confirming that MSTRG.6494 participates in wheat resistance to F. graminearum, may be via targeting TaATP2 to enhance defense responses. Our findings provide new insights into the regulatory mechanism of lincRNAs for Fusarium head blight (FHB) resistance, suggesting this mechanism as an essential strategy for protecting wheat from F. graminearum.

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