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Comprehensive analysis of histophysiology, transcriptome and metabolome tolerance mechanisms in black porgy ( Acanthopagrus schlegelii ) under low temperature stress

文献类型： 外文期刊

作者： Wang, Yue 1 ; Shen, Mingjun 1 ; Xu, Guangping 2 ; Yu, Han 1 ; Jia, Chaofeng 2 ; Zhu, Fei 2 ; Meng, Qian 2 ; Xu, Dafeng 2 ; Du, Shuran 2 ; Zhang, Dianchang 1 ; Zhang, Zhiwei 1 ;

作者机构： 1.Shanghai Ocean Univ, Natl Demonstrat Ctr Expt Fisheries Sci Educ, Shanghai 201306, Peoples R China

2.Jiangsu Marine Fishery Res Inst, Nantong 226007, Peoples R China

3.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangzhou 510300, Peoples R China

关键词： Black porgy(Acanthopagrusschlegelii); Low temperature stress; Histophysiology; Transcriptomics; Metabolomics

期刊名称：SCIENCE OF THE TOTAL ENVIRONMENT （ 影响因子：9.8； 五年影响因子：9.6 ）

ISSN： 0048-9697

年卷期： 2024 年 927 卷

页码：

收录情况： SCI

摘要： Low temperature stress has adverse effects on fish growth and reproduction, causing huge economic losses to the aquaculture industry. Especially, black porgy ( Acanthopagrus schlegelii ) farming industry in north of Yangtze River has been severely affected by low temperature for a long time. To explore the tolerance mechanism of black porgy to low temperature stress, the experiment was designed. The liver and gill tissues of black porgy were taken from the water temperature point of 15 degrees C (control group named as CG), 3.8 degrees C (cold sensitive group named as CS) and 2.8 degrees C (cold tolerant group named as CT) with a cooling rate of 3 degrees C/d from 15 degrees C for histophysiology, transcriptomics and metabolomics analysis. After cold stress, the histological results showed that the nucleus of the black porgy liver tissue appeared swelling, the cell arrangement was disordered; meanwhile the gill lamellae were twisted and broken, the epidermis was detached and aneurysm appeared. In addition, the expression of antioxidant, glucose metabolism and immune -related enzymes in the liver and gill of black porgy also changed significantly after low temperature stress. By analyzing the transcriptome and metabolome dates of black porgy liver, 3474 differentially expressed genes (DEGs) and 689 differentially expressed metabolites (DEMs) involved in low temperature stress were identified, respectively. The results of the transcriptome and metabolome combined analysis showed that individuals in the CS group mainly supplied energy to the body through lipid metabolism and amino acid metabolism, and meanwhile the apoptosis pathway was activated. While, individuals in the CT group mainly through glucose metabolism and steroid hormone biosynthesis to supply energy for the body. The validation results of qPCR on eight functional genes further demonstrated the reliability of RNA-Seq data. In summary, the results provide molecular information about adaptation to climate change and genetic selection of black porgy.

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