Improving Ultra-Low Temperature Preservation Technologies of Soybean Pollen for Off-Season and Off-Site Hybridization
文献类型： 外文期刊
作者： Jia, Hongchang 1 ; Liang, Xin 1 ; Zhang, Lixin 1 ; Zhang, Jinmei 1 ; Sapey, Enoch 1 ; Liu, Xianyuan 2 ; Sun, Yanhui 1 ; Sun, Shi 1 ; Yan, Hongrui 2 ; Lu, Wencheng 2 ; Han, Tianfu 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Crop Sci, Beijing, Peoples R China
2.Heilongjiang Acad Agr Sci, Heihe Branch, Heihe, Peoples R China
3.Council Sci & Ind Res CSIR, Oil Palm Res Inst, Kade, Ghana
关键词： soybean; pollen; ultra-low temperature preservation; germination rate; off-site hybridization
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：6.627； 五年影响因子：7.255 ）
ISSN： 1664-462X
年卷期： 2022 年 13 卷
页码：
收录情况： SCI
摘要： Preserving viable pollen is of great interest to breeders to maintain desirable germplasm for future inbreeding. Ultra-low temperature preservation of pollen is an effective and safe way for long-term storage of plant germplasm resources. In this study, we improved methods for the preservation of soybean pollen at ultra-low temperature. Soybean flowers at the initially-open stage were collected at 6-10 a.m. during the fully-bloom stage of soybean plants and were dehydrated for 10 h and then frozen and stored at -196 or -80 degrees C. In vitro culture experiments showed that the viability of preserved pollen remained as high as about 90%. The off-season (local site Heihe) and off-site (Beijing, after long-distance express delivery from Heihe) hybridization verification was conducted, and no significant difference in true hybrid rate was founded between the preserved pollen and the fresh pollen. The ultra-low temperature preservation technology for soybean pollen could break the spatiotemporal limit of soybean hybridization and facilitate the development of engineered soybean breeding.
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