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Molecular characterization and functional analysis of cytochrome P450-mediated detoxification CYP302A1 gene involved in host plant adaptation in Spodoptera frugieprda

文献类型： 外文期刊

作者： Hafeez, Muhammad 1 ; Li, Xiaowei 3 ; Chen, Limin 3 ; Ullah, Farman 5 ; Huang, Jun 1 ; Zhang, Zhijun 3 ; Zhang, Jinming 3 ; Siddiqui, Junaid Ali 6 ; Zhou, Shu-xing 3 ; Ren, Xiao-yun 3 ; Imran, Muhammad 7 ; Assiri, Mohammed A. 7 ; Lou, Yonggen 1 ; Lu, Yaobin 3 ;

作者机构： 1.Zhejiang Univ, Inst Insect Sci, State Key Lab Rice Biol, Minist Agr,Key Lab Mol Biol Crop Pathogens & Insec, Hangzhou, Peoples R China

2.Zhejiang Univ, Minist Agr, Inst Insect Sci, Key Lab Mol Biol Crop Pathogens & Insects, Hangzhou, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou, Peoples R China

4.Lishui Acad Agr & Forestry Sci, Integrated Plant Protect Ctr, Lishui, Peoples R China

5.China Agr Univ, Coll Plant Protect, Dept Plant Biosecur, Beijing, Peoples R China

6.Guizhou Univ, Coll Agr, Coll Tobacco Sci, Guiyang, Peoples R China

7.King Khalid Univ, Fac Sci, Dept Chem, Abha, Saudi Arabia

关键词： P450 detoxification enzyme; spodoptera frugiperda; CYP302a1; host adaptation; RNAi

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）

ISSN： 1664-462X

年卷期： 2023 年 13 卷

页码：

收录情况： SCI

摘要： The fall armyworm (FAW) Spodoptera frugiperda is a destructive and polyphagous pest of many essential food crops including maize and rice. The FAW is hard to manage, control, or eradicate, due to its polyphagous nature and voracity of feeding. Here, we report the characterization and functional analysis of the detoxification gene CYP302A1 and how S. frugieprda larvae use a detoxification mechanism to adapt host plants. Results demonstrated that CYP302A1 expression levels were much higher in midgut tissue and the older S. frugiperda larvae. Our current studies revealed the enhanced P450 activity in the midguts of S. frugiperda larvae after exposure to rice plants as compared to corn plants and an artificial diet. Furthermore, higher mortality was observed in PBO treated larvae followed by the exposure of rice plants as compared to the corn plant. The dsRNA-fed larvae showed downregulation of CYP302A1 gene in the midgut. At the same time, higher mortality, reduced larval weight and shorter developmental time was observed in the dsRNA-fed larvae followed by the exposure of rice plant as compared to the corn plant and DEPC-water treated plants as a control. These results concluded that the inducible P450 enzyme system and related genes could provide herbivores with an ecological opportunity to adapt to diverse host plants by utilizing secondary compounds present in their host plants.

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