文献类型： 外文期刊
作者： Ziye, Shang 1 ; Yuqiang, Zhao 4 ; Shitong, Wu 1 ; Ling, Cai 1 ; Chenchao, Sun 1 ; Jun, Wang 5 ; Weirong, Gong 6 ; Yanli, Tian 1 ; Baishi, Hu 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Plant Protect, Nanjing, Peoples R China
2.Nanjing Agr Univ, Key Lab Integrated Management Crop Dis & Pests, Nanjing, Peoples R China
3.Minist Agr & Rural Affairs, Key Lab Plant Quarantine Pests Monitoring & Contro, Nanjing, Peoples R China
4.Jiangsu Prov & Chinese Acad Sci, Nanjing Bot Garden Mem Sun Yat Sen, Inst Bot, Nanjing, Peoples R China
5.Plant Protect & Quarantine Stn Prov, Urumqi, Peoples R China
6.Plant Protect & Quarantine Stn Prov, Nanjing, Peoples R China
7.Xinjiang Acad Agr Sci, Inst Plant Protect, Xinjiang Key Lab Agr Biosafety, Key Lab Integrated Pest Management Crops Northwest, Urumqi, Xinjiang, Peoples R China
关键词： Paracidovorax citrulli; ClpA; RepA; virulence; regulate
期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：4.0； 五年影响因子：5.1 ）
ISSN：
年卷期： 2024 年 15 卷
页码：
收录情况： SCI
摘要： ClpA is a widely conserved protease in bacteria that plays a key role in virulence. To investigate its specific mechanism of action in the pathogenicity of Paracidovorax citrulli (formerly Acidovorax citrulli), we constructed a ClpA deletion mutant, Delta ClpA. The Delta ClpA mutant of P. citrulli displayed reduced virulence on melon seedlings, and reduced motility, swarming ability, and antioxidant capacity. On the other hand, the ClpA deletion of P. citrulli mutant reduced the resistance to elevated temperature and enhanced biofilm formation ability. Using qRT-PCR, we observed that ClpA negatively regulates the expression of the virulence-related genes virB, pilR, pilA, and fliM, while positively regulating hrpG, hrcQ, and trbC. Bacterial double hybrid and Glutathione-S-transferase pulldown (GST-pulldown) results showed that ClpA interacts directly with RepA, and negatively regulates the expression of RepA. After deletion of the RepA gene, the pathogenicity of P. citrulli was lost, biofilm formation ability was enhanced, and the expression of hrpG, pilR, and trbC was positively regulated. These results indicate that ClpA plays a key role in the regulation of several virulence traits of P. citrulli, paving the way for future studies to better elucidate the virulence mechanisms of this bacterial plant pathogen.
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