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Multiomics studies with co-transformation reveal microRNAs via miRNA-TF-mRNA network participating in wood formation in Hevea brasiliensis

文献类型： 外文期刊

作者： Chen, Jinhui 1 ; Liu, Mingming 1 ; Meng, Xiangxu 1 ; Zhang, Yuanyuan 4 ; Wang, Yue 1 ; Jiao, Nanbo 1 ; Chen, Jianmiao 1 ;

作者机构： 1.Hainan Univ, Sanya Nanfan Res Inst, Hainan Yazhou Bay Seed Lab, Key Lab Genet & Germplasm Innovat Trop Special For, Sanya, Peoples R China

2.Hainan Univ, Engn Res Ctr Rare & Precious Tree Species Hainan P, Sch Forestry, Haikou, Peoples R China

3.Hainan Univ, Sch Trop Crops, Haikou, Peoples R China

4.Chinese Acad Trop Agr Sci, Rubber Res Inst, Haikou, Hainan, Peoples R China

5.State Ctr Rubber Breeding, Haikou, Hainan, Peoples R China

关键词： reaction wood; phenylpropanoid biosynthesis pathway; lignin biosynthesis; Hevea brasiliensis; miRNA

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）

ISSN： 1664-462X

年卷期： 2023 年 14 卷

页码：

收录情况： SCI

摘要： Introduction: MicroRNAs (miRNAs) are small endogenous non-coding RNAs that play an important role in wood formation in plants. However, the significance of the link between miRNAs and their target transcripts in wood formation remains unclear in rubber tree (Hevea brasiliensis). Methods: In this study, we induced the formation of reaction wood by artificially bending rubber trees for 300 days and performed small RNA sequencing and transcriptome deep sequencing (RNA-seq) to describe the complement of miRNAs and their targets contributing to this process. Results and discussion: We identified 5, 11, and 2 differentially abundant miRNAs in normal wood (NW) compared to tension wood (TW), in NW relative to opposite wood (OW), and between TW and OW, respectively. We also identified 12 novel miRNAs and 39 potential miRNA-mRNA pairswith different accumulation patterns in NW, TW, and OW. We noticed that many miRNAs targeted transcription factor genes, which were enriched in KEGG pathways associated with phenylpropanoid biosynthesis, phenylalanine metabolism, and pyruvate metabolism. Thus, miRNA-TF-mRNA network involved in wood formation via tension wood model were constructed. We validated the differential accumulation of miRNAs and their targets by RT-qPCR analysis and overexpressed miRNA in Nicotiana benthamiana with its potential target gene. These results will provide a reference for a deep exploration of growth and development in rubber tree.

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