Antibacterial and Antibiofilm Effect of Ultrasound and Mild Heat Against a Multidrug-Resistant Klebsiella pneumoniae Stain Isolated from Meat of Yellow-Feathered Chicken
文献类型： 外文期刊
作者： Sun, Jinyue 1 ; Wang, Debao 2 ; Zhang, Jiaojiao 2 ; Sun, Zhilan 2 ; Xiong, Qiang 1 ; Liu, Fang 2 ;
作者机构： 1.Nanjing Tech Univ, Coll Food Sci & Light Ind, Nanjing, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Agr Prod Proc, Nanjing, Peoples R China
关键词： Klebsiella; biofilm; ultrasound; mild heat; dehydrogenase
期刊名称：FOODBORNE PATHOGENS AND DISEASE （ 影响因子：3.788； 五年影响因子：3.583 ）
ISSN： 1535-3141
年卷期：
页码：
收录情况： SCI
摘要： Klebsiella pneumoniae is an important foodborne pathogen with high biofilm-forming ability, which is continuously detected in food products in recent years. The antibacterial and antibiofilm activities and mechanism of ultrasonication in combination with heat treatment against K. pneumoniae were studied. K. pneumoniae planktonic and biofilm cells were treated with ultrasound (US), mild heat treatment (HT50, HT60, and HT70), and combinations of US and mild heat treatment (UH50, UH60, and UH70) for 5, 10, 20, 30, and 60 min. Results showed that the combination of US and mild heat treatment was more effective in inactivating K. pneumoniae planktonic and biofilm cells than the single treatment by counting viable bacteria. In addition, confocal laser scanning microscopy, scanning electron microscopy, and analysis of leakage of intracellular substances have revealed that the combination treatment effectively damaged the integrity of bacterial cell membrane and increased cell permeability, which led to the quick release of adenosine triphosphate (ATP) and macromolecular substances of nucleic acids and proteins. Moreover, the activities of respiratory chain dehydrogenase in planktonic and biofilm cells significantly decreased after UH treatment. The results indicated that ultrasonication and mild heat treatment had a synergistic effect on the inactivation of K. pneumoniae planktonic and biofilm cells by damaging the cell membrane and inhibiting intercellular cell respiration.
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