Major Bitter-Tasting Compounds from the Dichloromethane Fraction of Bitter Gourd (Fruit of Momordica charantia L.) Extract and Their Precursors
文献类型： 外文期刊
作者： Cai, Xueyi 1 ; Lin, Ziqiang 1 ; Zheng, Qihong 1 ; Liao, Mengzhen 1 ; Li, Hongfu 1 ; Feng, Hanxiao 2 ; Chen, Huan 1 ; Zhang, Yang 2 ; Chen, Xiao 3 ; Liang, Dong 1 ;
作者机构： 1.Sun Yat Sen Univ, Sch Agr & Biotechnol, Shenzhen 518107, Peoples R China
2.Sun Yat Sen Univ, Sch Publ Hlth Shenzhen, Shenzhen 518107, Peoples R China
3.Guangdong Acad Agr Sci, Vegetable Res Inst, Guangdong Key Lab New Technol Res Vegetables, Guangzhou 501640, Peoples R China
关键词： Momordica charantia L.; bitter gourd; bittertaste; cucurbitane; dose-over-threshold; isomerization; sensomics
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）
ISSN： 0021-8561
年卷期： 2024 年 72 卷 40 期
页码：
收录情况： SCI
摘要： The unripe fruit of the plant Momordica charantia L., commonly known as bitter gourd or bitter melon, is a popular vegetable and medical herb in many parts of the world and is characterized by its strong bitter taste. In our endeavor to decode its bitter taste, the dichloromethane fraction of 75% methanol extract of bitter gourd was found to be intensely bitter. Combining sensory analysis-guided fractionation and newly developed comparative high-pressure liquid chromatography (HPLC) analysis-guided purification led to the isolation of five known compounds including momordicoside L ( 1 ), (23E)-3 beta-O-malonyl-7 beta,25-dihydroxycucurbita-5,23-dien-19-al ( 2 ), 3-O-beta-d-allopyranosyl-7 beta,25-dihydroxycucurbita-5,23(E)-dien-19-al ( 3 ), momordicine IV ( 5 ), and charantoside B ( 6 ) and three new compounds 3-O-beta-d-3-ketoglucopyranosly-7 beta,25-dihydroxycucurbita-5,23(E)-dien-19-al ( 4 ), 6 '-O-malonylmomordicoside L ( 7 ), and 6 '-O-malonylmomordicine IV ( 8 ) from bitter gourd. Sensory analysis revealed compounds 3 - 8 had strong bitter taste with their bitter taste recognition thresholds in the range between 3.6 ( 4 ) and 13.6 ppm ( 6 ) in 3% ethanol. UPLC-MS/MS quantification showed that their concentration in bitter gourd ranged from 16.5 +/- 1.3 mg/kg ( 4 ) to 214.8 +/- 14.0 mg/kg ( 6 ) on a dry weight basis. Calculation of the dose-over-threshold (DoT) factor showed that momordicine IV ( 5 ) and charantoside B ( 6 ) should be considered as bitter principles of bitter gourd. In addition, the study also demonstrated the ubiquity of the isomerization reaction in the side chain of cucurbitane-type triterpenoids. Many isolated compounds were the isomerized products of their natural precursors, and these precursors should be the primary bitterness contributors of fresh fruits. In addition, comparative HPLC analysis-guided purification could be a practical approach for the fast isolation of acid-labile precursors.
- 相关文献
作者其他论文 更多>>
-
Multifunctional Role of Cytokinin in Horticultural Crops
作者：Hussain, Shahid;Chang, Jingjing;Li, Jing;Chen, Lei;Song, Zhao;Zhang, Baige;Chen, Xiao;Ahmad, Sheraz
关键词：cytokinin; flowering; fruit setting; stress biology; physiology
-
Discovery of major bitter-tasting compounds from bitter gourd (fruit of Momordica charantia L.) by application of the sensomics approach
作者：Cai, Xueyi;Li, Hongfu;Liao, Mengzhen;Zheng, Qihong;Chen, Huan;Liang, Dong;Cai, Xueyi;Zhang, Yang;Chen, Xiao
关键词：Bitter gourd; Momordica charantia L.; Bitter taste; Sensomics; Taste dilution analysis; Taste recognition threshold
-
Biochar plays multiple roles in BPA/persulfate degradation system
作者：Yi, Xing;Chen, Xiao;Jiang, Shaojun;Shu, Yuehong;Jiang, Shaojun
关键词：Biochar; Organic matter; Persulfate; Bisphenol A; Advanced oxidation
-
Rethinking the Cucurbitane-Type Triterpenoid Profile of Bitter Gourd (Momordica charantia L.): The Chemical Transformation of Momordicine I in Solution
作者：Cai, Xueyi;Lin, Ziqiang;Liu, Lang;Liang, Dong;Cai, Xueyi;Lin, Ziqiang;Liu, Lang;Zhang, Yang;Yan, Wangwang;Wang, Zi;Chen, Xiao;Liang, Dong
关键词：
Momordica charantia L .; bitter gourd; cucurbitane; triterpenoid; 5,19-hemiacetal; acetal; dietary supplement -
Calcium deficiency leads to fruit blackheart formation by disrupting glycometabolism and phenylpropanoid metabolism in wax gourd
作者：Chang, Jingjing;Li, Jinlong;Li, Jing;Li, Zhen;Chen, Xiao;Song, Zhao;Zhang, Baige;Liao, Daolong;Zhang, Baige
关键词：Storage quality; Ca; Pectin degradation; Monosaccharides; Metabolic and transcriptomic analysis
-
Transcriptome Wide Identification and Expression Analysis Revealed BhTALE Gene Family Regulates Wax Gourd (Benincasa hispida) Response to Low Calcium and Magnesium Stress
作者：Hussain, Shahid;Chang, Jingjing;Li, Jing;Chen, Xiao;Xie, Dasen;Zhang, Baige
关键词：
Benincasa hispida ; low calcium; low magnesium;TALE -
Melatonin Enhances the Low-Calcium Stress Tolerance by Regulating Brassinosteroids and Auxin Signals in Wax Gourd
作者：Chang, Jingjing;Zhu, Xuemei;Lian, Yixuan;Li, Jing;Chen, Xiao;Song, Zhao;Chen, Lei;Xie, Dasen;Zhang, Baige
关键词：low-Ca stress; melatonin; brassinosteroid; auxin; wax gourd
