Transcriptome Analysis in Haematococcus pluvialis: Astaxanthin Induction by Salicylic Acid (SA) and Jasmonic Acid (JA)
文献类型： 外文期刊
作者： Gao, Zhengquan 1 ; Li, Yan 3 ; Wu, Guanxun 1 ; Li, Guoqiang 1 ; Sun, Haifeng 1 ; Deng, Suzhen 1 ; Shen, Yicheng 1 ; Chen, 1 ;
作者机构： 1.Shandong Univ Technol, Sch Life Sci, Zibo 255049, Peoples R China
2.Chinese Acad Fishery Sci, Yellow Sea Fishery Res Inst, Qingdao 266071, Peoples R China
3.James Cook Univ, Coll Marine & Environm Sci, Douglas 4811, Australia
4.Univ Queensland, Sch Food & Agr Sci, St Lucia, Qld 4072, Australia
期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）
ISSN： 1932-6203
年卷期： 2015 年 10 卷 10 期
页码：
收录情况： SCI
摘要： Haematococcus pluvialis is an astaxanthin-rich microalga that can increase its astaxanthin production by salicylic acid (SA) or jasmonic acid (JA) induction. The genetic transcriptome details of astaxanthin biosynthesis were analyzed by exposing the algal cells to 25 mg/L of SA and JA for 1, 6 and 24 hours, plus to the control (no stress). Based on the RNA-seq analysis, 56,077 unigenes (51.7%) were identified with functions in response to the hormone stress. The top five identified subcategories were cell, cellular process, intracellular, catalytic activity and cytoplasm, which possessed 5600 (similar to 9.99%), 5302 (similar to 9.45%), 5242 (similar to 9.35%), 4407 (similar to 7.86%) and 4195 (similar to 7.48%) unigenes, respectively. Furthermore, 59 unigenes were identified and assigned to 26 putative transcription factors (TFs), including 12 plant-specific TFs. They were likely associated with astaxanthin biosynthesis in Haematococcus upon SA and JA stress. In comparison, the up-regulation of differential expressed genes occurred much earlier, with higher transcript levels in the JA treatment (about 6 h later) than in the SA treatment (beyond 24 h). These results provide valuable information for directing metabolic engineering efforts to improve astaxanthin biosynthesis in H. pluvialis.
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