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Molecular cloning and structural characterization of PPAR alpha gene from turbot (Scophthalmus maximus) and functional exploration of lipid metabolism in response to thermal stress

文献类型： 外文期刊

作者： Zhao, Tingting 1 ; Ma, Aijun 1 ; Huang, Zhihui 1 ; Liu, Zhifeng 1 ; Wang, Xinan 1 ; Sun, Zhibin 1 ; Yang, Jingkun 1 ; Li, Yingdi 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao Key Lab Marine Fish Breeding & Biotechnol, Shandong Key Lab Marine Fisheries Biotechnol & Ge, 106 Nanjing Rd, Qingdao 266071, Shandong, Peoples R China

2.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China

3.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Minist Educ, Shanghai, Peoples R China

关键词： lipid metabolism; PPAR alpha; Scophthalmus maximus; thermal stress

期刊名称：AQUACULTURE RESEARCH （ 影响因子：2.184； 五年影响因子：2.447 ）

ISSN： 1355-557X

年卷期： 2022 年 53 卷 7 期

页码：

收录情况： SCI

摘要： Peroxisome proliferator-activated receptor alpha (PPAR alpha), as a transcription factor, plays an important role in the regulation of lipid metabolism. To better understand the regulatory role of PPAR alpha in lipid metabolism in turbot, we analyzed the sequence structure of PPAR alpha and its stress response to a high temperature. We also investigated the regulation of genes related to lipid metabolism after the knockdown of PPAR alpha. The CDS of PPAR alpha was 1410 bp, encoding 469 amino acids. The relative molecular weight of the protein was 52.64 kDa, and the predicted pI was 5.63. The architecture of the PPAR alpha gene exhibited a ZnF-C4 domain and a HOLI domain. Phylogenetic analysis indicated that PPAR alpha has been highly conserved during evolution. The qPCR results showed that the mRNA level of PPAR alpha was significantly increased, and the expression of the lipid synthesis genes FASN and SCD was significantly downregulated under high-temperature stress. PPAR alpha interference and inhibition results showed that the expression levels of FASN and SCD were significantly increased. Our results suggest that high temperatures activate PPAR alpha and inhibit lipid synthesis by downregulating the expression of FASN and SCD in order to reduce lipid deposition and maintain lipid metabolism disorder caused by high-temperature stress. This is the first report of PPAR alpha in turbot. It will help for studying the evolution of PPAR alpha and provide a reliable reference for studying the regulation mechanism of lipid metabolism in fish under stress.

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