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Prime Editing of TaEPSPS Homeologs Enhances Wheat Resistance to Glyphosate

文献类型： 外文期刊

作者： Chen, Tianzi 1 ; Li, Yaning 3 ; Qiu, Zeyu 1 ; Zou, Changsong 3 ; Zhang, Baolong 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Prov Key Lab Agrobiol, Nanjing 210014, Peoples R China

2.Zhongshan Biol Breeding Lab, Nanjing 210014, Peoples R China

3.Henan Univ, Sch Life Sci, Natl Key Lab Cotton Biobreeding & Integrated Utili, Kaifeng 475001, Peoples R China

关键词： Wheat; EPSPS gene; prime editing; glyphosate resistance

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.2； 五年影响因子：6.4 ）

ISSN： 0021-8561

年卷期： 2025 年 73 卷 34 期

页码：

收录情况： SCI

摘要： 5-Enolpyruvylshikimate-3-phosphate synthase (EPSPS) is the primary target of the broad-spectrum herbicide glyphosate, and several well-characterized amino acid substitutions in EPSPS are known to confer glyphosate resistance. Here, we introduced the P106S substitution into wheat TaEPSPS via prime editing, resulting in resistance to the field-recommended dosage of glyphosate. Either homozygous or heterozygous P106S in a single TaEPSPS homeolog was sufficient to confer resistance, with tolerance levels of 461 g a.e. ha-1 at 4 degrees C and 1383-1845 g a.e. ha-1 at 28 degrees C. The P106S substitution reduced shikimate accumulation by 60-77% under glyphosate treatment, did not alter the native expression patterns of TaEPSPS, and incurred negligible fitness costs under nonglyphosate conditions. Although P106S-edited lines exhibited growth inhibition and an approximate 10-day growth delay following glyphosate application, no significant yield penalty was observed at maturity. These glyphosate-resistant P106S alleles hold promising potential for integration into breeding programs to enhance weed control strategies.

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