High density cellulose nanofibril assembly leads to upgraded enzymatic and chemical catalysis of fermentable sugars, cellulose nanocrystals and cellulase production by precisely engineering cellulose synthase complexes
文献类型： 外文期刊
作者： Zhang, Ran 1 ; Hu, Zhen 1 ; Peng, Hao 1 ; Liu, Peng 1 ; Wang, Youmei 1 ; Li, Jingyang 2 ; Lu, Jun 3 ; Wang, Yanting 1 ; Xia, Tao 2 ; Peng, Liangcai 1 ;
作者机构： 1.Hubei Univ Technol, Natl Ctr Cellular Regulat & Mol Pharmaceut 111, Cooperat Innovat Ctr Ind Fermentat, Key Lab Fermentat Engn,Hubei Key Lab Ind Microbiol, Wuhan 430068, Peoples R China
2.Huazhong Agr Univ, Coll Plant Sci & Technol, Biomass & Bioenergy Res Ctr, Wuhan 430070, Peoples R China
3.Hubei Univ Arts & Sci, Coll Food Sci & Chem Engn, Lab Biomass Engn & Nanomat Applicat Automobiles, Xiangyang 441003, Peoples R China
4.Huazhong Agr Univ, Coll Resources & Environm, Wuhan 430070, Peoples R China
5.Chinese Acad Trop Agr Sci, Haikou Expt Stn, Haikou 570102, Peoples R China
6.Huazhong Agr Univ, Coll Life Sci & Technol, Wuhan 430070, Peoples R China
期刊名称：GREEN CHEMISTRY （ 影响因子：9.8； 五年影响因子：9.8 ）
ISSN： 1463-9262
年卷期： 2023 年 25 卷 3 期
页码：
收录情况： SCI
摘要： Although cellulose represents the most abundant biomass resource on Earth, its natural recalcitrance hinders its application for low-cost biofuels and high-value bioproducts on a large scale. In this study, we generated less recalcitrant cellulose nanofibril substrates by site-specific mutations and knockouts of three OsCESA4,7,9 isoforms that are essential as cellulose synthase complexes for the cellulose biosynthesis of secondary cell walls in rice. As a comparison with the wild type form, three genetically-engineered CESAs isoforms independently generated cellulose nanofibrils with the average lengths reduced by 63%, which accounts for the high density of the amorphous cellulose chains as the initial breakpoints for the consistently enhanced biomass enzymatic hydrolysis into fermentable sugars. The genetically modified cellulose nanofibrils efficiently produce ideal nanocrystals with the most reduced dimension to-date in three knockout lines, which are applicable as optimal intermediates for highly valuable bioproducts. The improved cellulose nanofibrils are also effective in inducing the secretion of lignocellulose-degradation enzymes from fungi (T. reesei) with the two cellulase (exoglucanases, beta-glucosidases) activities elevated by 100% and 138%, and the total protein level increased by 44%. Our data thus reveal a novel green strategy for achieving high-quality diverse bioproduction by integrating the precise genetic modification of lignocellulose substrates with efficient biomass process technology.
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