Metabolic engineering of Paenibacillus polymyxa for effective production of 2,3-butanediol from poplar hydrolysate
文献类型： 外文期刊
作者： Zhang, Jikun 1 ; Zhao, Jianzhi 2 ; Fu, Quanbin 4 ; Liu, Haiyang 1 ; Li, Min 1 ; Wang, Zhongyue 1 ; Gu, Wei 6 ; Zhu, Xueming 5 ; Lin, Rongshan 1 ; Dai, Li 4 ; Liu, Kai 1 ; Wang, Chengqiang 1 ;
作者机构： 1.Shandong Agr Univ, Coll Life Sci, Natl Engn Res Ctr Efficient Utilizat Soil & Fertil, Shandong Engn Res Ctr Plant Microbia Restorat Sali, Tai An 271018, Peoples R China
2.Shandong Acad Sci, Qilu Univ Technol, Sch Bioengn, State Key Lab Biobased Mat & Green Papermaking, Jinan 250353, Peoples R China
3.State Key Lab Microbial Technol, Jinan 250353, Peoples R China
4.Shandong Agr Univ, Coll Chem & Mat Sci, Tai An 271018, Peoples R China
5.Zhejiang Acad Agr Sci, State Key Lab Managing Biot & Chem Treats Qual & S, Hangzhou 310021, Peoples R China
6.Shandong Baolai leelai Bioengn Co Ltd, Tai An 271000, Peoples R China
7.Shandong Agr Univ, Coll Life Sci, 61 Daizong St, Tai An, Peoples R China
关键词： Renewable fuel; Omics analysis; Metabolic pathway; Raw poplar wood hydrolysates
期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：11.4； 五年影响因子：10.6 ）
ISSN： 0960-8524
年卷期： 2024 年 392 卷
页码：
收录情况： SCI
摘要： 2,3-Butanediol is an essential renewable fuel. The synthesis of 2,3-butanediol using Paenibacillus polymyxa has attracted increasing attention. In this study, the glucose-derived 2,3-butanediol pathway and its related genes were identified in P. polymyxa using combined transcriptome and metabolome analyses. The functions of two distinct genes ldh1 and ldh3 encoding lactate dehydrogenase, the gene bdh encoding butanediol dehydrogenase, and the spore-forming genes spo0A and spoIIE were studied and directly knocked out or overexpressed in the genome sequence to improve the production of 2,3-butanediol. A raw hydrolysate of poplar wood containing 27 g/L glucose and 15 g/L xylose was used to produce 2,3-butanediol with a maximum yield of 0.465 g/g and 93 % of the maximum theoretical value, and the total production of 2,3-butanediol and ethanol reached 21.7 g/L. This study provides a new scheme for engineered P. polymyxa to produce renewable fuels using raw poplar wood hydrolysates.
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