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Cand2 inhibits CRL-mediated ubiquitination and suppresses autophagy to facilitate pathogenicity of phytopathogenic fungi

文献类型： 外文期刊

作者： Zhang, Yunran 1 ; Wei, Yunyun 4 ; Wu, Minghua 1 ; Liu, Mengyu 1 ; Liang, Shuang 3 ; Zhu, Xueming 3 ; Liu, Xiaohong 1 ; Lin, Fucheng 1 ;

作者机构： 1.Zhejiang Univ, Inst Biotechnol, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310058, Peoples R China

2.Zhejiang Univ, Hainan Inst, Sanya 572025, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Plant Protect & Microbiol, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Peoples R China

4.Zhejiang Shuren Univ, Coll Biol & Environm Engn, Hangzhou 310015, Peoples R China

关键词： Magnaporthe oryzae; autophagy; ubiquitination; pathogenicity; regulation mechanism; phytopathoogenic fungi

期刊名称：PLANT COMMUNICATIONS （ 影响因子：10.5； 五年影响因子：10.5 ）

ISSN： 2590-3462

年卷期： 2024 年 5 卷 2 期

页码：

收录情况： SCI

摘要： The ubiquitin-proteasome system and the autophagy system are the two primary mechanisms used by eukaryotes to maintain protein homeostasis, and both are closely related to the pathogenicity of the rice blast fungus. In this research, we identified MoCand2 as an inhibitor of ubiquitination in Magnaporthe oryzae. Through this role, MoCand2 participates in the regulation of autophagy and pathogenicity. Specifically, we found that deletion of MoCand2 increased the ubiquitination level in M. oryzae, whereas overexpression of MoCand2 inhibited the accumulation of ubiquitinated proteins. Interaction analyses showed that MoCand2 is a subunit of Cullin-RING ligases (CRLs). It suppresses ubiquitination by blocking the assembly of CRLs and downregulating the expression of key CRL subunits. Further research indicated that MoCand2 regulates autophagy through ubiquitination. MoCand2 knockout led to overubiquitination and over-degradation of MoTor, and we confirmed that MoTor content was negatively correlated with autophagy level. In addition, MoCand2 knockout accelerated the K63 ubiquitination of MoAtg6 and strengthened the assembly and activity of the phosphatidylinositol-3-kinase class 3 complex, thus enhancing autophagy. Abnormal ubiquitination and autophagy in DMocand2 resulted in defects in growth, conidiation, stress resistance, and pathogenicity. Finally, sequence alignment and functional analyses in other phytopathogenic fungi confirmed the high conservation of fungal Cand2s. Our research thus reveals a novel mechanism by which ubiquitination regulates autophagy and pathogenicity in phytopathogenic fungi.

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