文献类型： 外文期刊
作者： Ren, Hailong 1 ; Wei, Zhenwu 1 ; Zhou, Bo 3 ; Chen, Xiang 1 ; Gao, Qiang 3 ; Zhang, Zhibin 4 ;
作者机构： 1.Yangzhou Univ, Coll Anim Sci & Technol, Yangzhou, Jiangsu, Peoples R China
2.Guangzhou Acad Agr Sci, Guangzhou, Guangdong, Peoples R China
3.Xinjiang Acad Agr Sci, Hainan Sanya Test Ctr Crop Breeding, Sanya, Hainan, Peoples R China
4.Chinese Acad Agr Sci, Inst Cotton Res, State Key Lab Cotton Biol, Anyang, Henan, Peoples R China
关键词： Medicago polymorpha; SLAF-seq; Molecular marker development
期刊名称：PEERJ （ 影响因子：2.7； 五年影响因子：3.1 ）
ISSN： 2167-8359
年卷期： 2023 年 11 卷
页码：
收录情况： SCI
摘要： Medicago polymorpha L. (bur clover), an invasive plant species of the genus Medicago, has been traditionally used in China as an edible vegetable crop because of its high nutritive value. However, few molecular markers for M. polymorpha have been identified. Using the recently published high-quality reference genome of M. polymorpha, we performed a specific-locus amplified fragment sequencing (SLAF-seq) analysis of 10 M. polymorpha accessions to identify molecular markers and explore genetic diversity. A total of 52,237 high-quality single nucleotide polymorphisms (SNPs) were developed. These SNPs were mostly distributed on pseudochromosome 3, least distributed on pseudochromosome 7, and relatively evenly distributed on five other pseudochromosomes of M. polymorpha. Phenotypic analysis showed that there was a great difference in phenotypic traits among different M. polymorpha accessions. Moreover, clustering all M. polymorpha accessions based on their phenotypic traits revealed three groups. Both phylogenetic analysis and principal component analysis (PCA) of all M. polymorpha accessions based on SNP markers consistently indicated that all M. polymorpha accessions could be divided into three distinct groups (I, II, and III). Subsequent genetic diversity analysis for the 10 M. polymorpha accessions validated the effectiveness of the M. polymorpha germplasm molecular markers in China. Additionally, SSR mining analysis was also performed to identify polymorphic SSR motifs, which could provide valuable candidate markers for the further breeding of M. polymorpha. Since M. polymorpha genetics have not been actively studied, the molecular markers generated from our research will be useful for further research on M. polymorpha resource utilization and marker-assisted breeding.
- 相关文献
作者其他论文 更多>>
-
A prediction model of rubber content in the dried root of Taraxacum kok-saghyz Rodin based on near-infrared spectroscopy
作者：Chen, Runfeng;Zheng, Lipeng;Chen, Runfeng;Yan, Qingqing;Tuoheti, Tuhanguli;Xu, Lin;Gao, Qiang;Zhang, Yan;Zheng, Lipeng;Yan, Qingqing;Tuoheti, Tuhanguli;Xu, Lin;Gao, Qiang;Zhang, Yan;Ren, Hailong;Wang, Feng;Liu, Ya
关键词：Near-infrared spectroscopy; Taraxacum kok-saghyz; Natural rubber; Rapid detection; PLS; RF; LightGBM; CNN
-
Screening structure and predicting toxicity of pesticide adjuvants using molecular dynamics simulation and machine learning for minimizing environmental impacts
作者：Bao, Zhenping;Liu, Rui;Wu, Yanling;Zhang, Songhao;Gao, Yuxia;Zhang, Chenhui;Du, Fengpei;Luckham, Paul;Zhang, Xuejun;Zhou, Bo;Zhang, Xuejun;Zhou, Bo
关键词：Surfactants; Structure screening; Molecules dynamic simulation; Machine learning; Toxicity prediction; Pesticide adjuvants
-
The Applicability of Remote Sensing Models of Soil Salinization Based on Feature Space
作者：Liu, Jing;Zhang, Li;Dong, Tong;Fan, Yanmin;Wu, Hongqi;Zhang, Zhibin;Liu, Jing;Zhang, Li;Dong, Tong;Fan, Yanmin;Wu, Hongqi;Zhang, Zhibin;Liu, Jing;Wang, Juanle;Geng, Qinglong;Yang, Qiangjun
关键词：salinization; feature space; remote sensing inversion; coastal areas; arid areas; applicability
-
Salt-responsive transcriptome analysis of triticale reveals candidate genes involved in the key metabolic pathway in response to salt stress
作者：Deng, Chaohong;Shi, Shubing;Yan, Guorong;Wang, Fan;Zhao, Lianjia;Liu, Ning;Abudurezike, Abudukeyoumu;Li, Yushan;Wang, Wei;Zhang, Zhibin
关键词：
-
Comparative analysis of volatile compounds in thirty nine melon cultivars by headspace solid-phase microextraction and gas chromatography-mass spectrometry
作者：Shi, Jianda;Xiong, Mu;Bie, Zhilong;Huang, Yuan;Shi, Jianda;Xiong, Mu;Bie, Zhilong;Huang, Yuan;Wu, Haibo;Chen, Jihao;Zhou, Bo;Fu, Xiaofa;Chen, Yanjun;Wang, Hui;Li, Liangliang
关键词：Aroma volatiles; Cucumis melo; Fruit quality; HS-SPME-GC-MS
-
Genetic diversity and evolutionary patterns of Taraxacum kok-saghyz Rodin
作者：Zhang, Yan;Wang, Li;Abudurezike, Abudukeyoumu;Yan, Qingqing;Lu, Zifeng;Wang, Yonggang;Xu, Lin;Ren, Hailong;Zhang, Xuechao;Gao, Qiang;Nie, Qiuhai;Zhang, Zhibin
关键词：adaptation; population genetic diversity; population structure; rubber dandelion; SNP
