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Cloning and Functional Analysis of TaWRI1Ls, the Key Genes for Grain Fatty Acid Synthesis in Bread Wheat

文献类型： 外文期刊

作者： Yang, Fengping 1 ; Liu, Guoyu 1 ; Wu, Ziyan 1 ; Zhang, Dongxue 1 ; Zhang, Yufeng 1 ; You, Mingshan 1 ; Li, Baoyun 1 ; Zhang, Xiuhai 3 ; Liang, Rongqi 1 ;

作者机构： 1.China Agr Univ, Key Lab Crop Heterosis & Utilizat MOE, Beijing 100193, Peoples R China

2.China Agr Univ, Beijing Key Lab Crop Genet Improvement, Beijing 100193, Peoples R China

3.Beijing Acad Agr & Forestry Sci, Beijing 100097, Peoples R China

关键词： bread wheat; TaWRI1; fatty acid synthesis; functional analysis; transcription factor

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）

ISSN：

年卷期： 2022 年 23 卷 10 期

页码：

收录情况： SCI

摘要： WRINKLED1 (WRI1), an APETALA2 (AP2) transcription factor (TF), critically regulates the processes related to fatty acid synthesis, storage oil accumulation, and seed development in plants. However, the WRI1 genes remain unknown in allohexaploid bread wheat (Triticum aestivum L.). In this study, based on the sequence of Arabidopsis AtWRI1, two TaWRI1Ls genes of bread wheat, TaWRI1L1 and TaWRI1L2, were cloned. TaWRI1L2 was closely related to monocotyledons and clustered in one subgroup with AtWRI1, while TaWRI1L1 was clustered in another subgroup with AtWRI3 and AtWRI4. Both were expressed highly in the developmental grain, subcellular localized in the nucleus, and showed transcriptional activation activity. TaWRI1L2, rather than TaWRI1L1, promoted oil body accumulation and significantly increased triglyceride (TAG) content in tobacco leaves. Overexpression of TaWRI1L2 compensated for the functional loss of AtWRI1 in an Arabidopsis mutant and restored the wild-type phenotypes of seed shape, generation, and fatty acid synthesis and accumulation. Knockout of TaWRI1L2 reduced grain size, 1000 grain weight, and grain fatty acid synthesis in bread wheat. Conclusively, TaWRI1L2, rather than TaWRI1L1, was the key transcriptional factor in the regulation of grain fatty acid synthesis in bread wheat. This study lays a foundation for gene regulation and genetic manipulation of fatty acid synthesis in wheat genetic breeding programs.

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