您好，欢迎访问浙江省农业科学院 机构知识库！
浙江省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Molecular Detection and Genotyping of Toxoplasma gondii from Pigs for Human Consumption in Zhejiang and Jiangsu Provinces, Eastern China

文献类型： 外文期刊

作者： Sun, Hong-Chao 1 ; Fu, Yuan 1 ; Yuan, Xiu-Fang 1 ; Li, Jun-Xing 1 ; Xu, Li-hua 1 ; Zhang, Jia-Ning 3 ; Yu, Bin 1 ; Huang, Jing 4 ; Qi, Meng 3 ; Shi, Tuan-Yuan 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Med, Hangzhou, Peoples R China

2.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Med, Hangzhou 310021, Peoples R China

3.Tarim Univ, Coll Anim Sci, Alar, Peoples R China

4.Zhejiang Prov Anim Dis Prevent & Control Ctr, Hangzhou, Peoples R China

关键词： Toxoplasma gondii; pigs; prevalence; genetic characterization; China

期刊名称：FOODBORNE PATHOGENS AND DISEASE （ 影响因子：3.788； 五年影响因子：3.583 ）

ISSN： 1535-3141

年卷期：

页码：

收录情况： SCI

摘要： Toxoplasma gondii infections are common in humans and animals worldwide. Ingestion of raw or undercooked meat containing tissue cysts of T. gondii is one major source of transmission of this parasite. It is important to guarantee the meat quality of China since our pork industry produces about half of the world's pork. In this study, a total of 746 pig samples were collected from Zhejiang and Jiangsu provinces in eastern China, and examined for T. gondii infection by PCR amplification targeting B1 gene. In this study, we found that 57 of 746 (7.6%) pigs were positive for B1 gene, with 8.5% (48/562) in Zhejiang province and 4.9% (9/184) in Jiangsu province, respectively. The positive DNA samples were further genotyped at 11 genetic markers, including SAG1, 5 '-and 3 '-SAG2, alternative SAG2, SAG3, BTUB, GRA6, L358, PK1, c22-8, c29-2, and an apicoplast locus Apico through PCR-restriction fragment length polymorphism (PCR-RFLP) technology. Two genotypes (ToxoDB 9 and ToxoDB 10) of T. gondii were identified by PCR-RFLP in Zhejiang province. However, both genotypes were not determined from Jiangsu province, which is speculated on the low DNA concentration and the small number of samples. These results indicate that T. gondii infection is endemic in pigs in eastern China and may raise public food safety concerns, suggesting more interventions for T. gondii-related risks are needed in the future.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：浙江省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台