Identification of a novel promoter region responsible for the embryo-specific expression of SERK1 in pineapple
文献类型： 外文期刊
作者： Xie, Tao 1 ; Zhang, Wei 2 ; Chen, Chengjie 2 ; Wang, Xiaoshuang 2 ; Zhang, Jing 1 ; Luan, Aiping 3 ; He, Yehua 2 ;
作者机构： 1.Foshan Univ, Dept Hort, Foshan 528231, Peoples R China
2.South China Agr Univ, Key Lab Biol & Germplasm Enhancement Hort Crops So, Minist Agr, Guangzhou 510642, Peoples R China
3.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Haikou 571101, Peoples R China
4.South China Agr Univ, Coll Hort, Guangzhou 510642, Peoples R China
关键词： Pineapple; AcSERK1; Somatic embryogenesis; Embryogenic callus; Embryonic cell-specific region; Promoter analysis
期刊名称：HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY （ 影响因子：2.4； 五年影响因子：2.5 ）
ISSN： 2211-3452
年卷期： 2023 年
页码：
收录情况： SCI
摘要： Somatic embryogenesis (SE) is a key regeneration process in plant. AcSERK1 is a gene specifically expressed in the early stage of SE in pineapple (Ananas comosus), suggesting that the promoter of SERK1 might contain specific cis-acting element regulating SE. To identify embryonic cell-specific element in the SERK1 promoter, a series of binary plant transformation vectors with GUS (beta-glucuronidase) reporter gene were systematically analyzed by transient gene expression system in wild-type and transgenic pineapple embryogenic callus. Histochemical and quantitative GUS assays demonstrated that the activity of the AcSERK1 upstream regulatory sequence lacking - 921 to -911 or -910 to -880 was significantly reduced in the embryonic callus of the pineapple, and these two regions were needed for the embryonic cell-specific. Besides, a promoter lacking - 943 to -922 was shown to significantly increase GUS activity in embryogenic callus, suggesting repressive elements exist in this region. Our data of stable transformation assays confirmed again the 5' upstream regulatory sequence (-921 to -880) of the AcSERK1 gene is an essential functional region. Our findings lay the basis for better understanding of the molecular mechanisms of AcSERK1 gene in the regulation in early stage of SE.
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