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Comprehensive analysis of Dendrobium catenatum HSP20 family genes and functional characterization of DcHSP20-12 in response to temperature stress

文献类型： 外文期刊

作者： Wang, Peng 1 ; Zhang, Tingting 3 ; Li, Yuxin 2 ; Zhao, Xi 2 ; Liu, Wen 1 ; Hu, Yanping 2 ; Wang, Jian 1 ; Zhou, Yang 1 ;

作者机构： 1.Hainan Univ, Sanya Inst Breeding & Multiplicat, Sanya 572025, Hainan, Peoples R China

2.Hainan Univ, Key Lab Qual Regulat Trop Hort Crops Hainan Prov, Sch Trop Agr & Forestry, Sch Agr & Rural Affairs,Sch Rural Revitalizat, Haikou, Hainan, Peoples R China

3.Xiangyang Acad Agr Sci, Xiangyang 441057, Hubei, Peoples R China

4.Hainan Acad Agr Sci, Inst Vegetables, Hainan Vegetable Breeding Engn Technol Res Ctr, Key Lab Vegetable Biol Hainan Prov, Haikou 571199, Hainan, Peoples R China

5.Hainan Univ, 58 Renmin Rd, Haikou 570228, Peoples R China

关键词： Dendrobium catenatum; HSP20 gene family; High temperature stress; Low temperature stress

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.2； 五年影响因子：7.8 ）

ISSN： 0141-8130

年卷期： 2024 年 258 卷

页码：

收录情况： SCI

摘要： Heat shock proteins (HSPs) are a class of protective proteins in response to abiotic stress in plants, and HSP20 plays an essential role in response to temperature stress. However, there are few studies on HSP20 in Dendrobium catenatum. In this study, 18 DcHSP20 genes were identified from the D. catenatum genome. Phylogenetic analysis showed that DcHSP20s could be classified into six subgroups, each member of which has similar conserved motifs and gene structures. Gene expression analysis of 18 DcHSP20 genes revealed that they exhibited variable expression patterns in different plant tissues. Meanwhile, all 18 DcHSP20 genes were induced to be up-regulated under high temperature, while six genes (DcHSP20-2/9/10/12/16/17) were significantly up-regulated under low temperature. Moreover, combining gene expression under high and low temperature stress, the DcHSP20-12 gene was cloned for functional analysis. The germination ratios, fresh weights, root lengths of two DcHSP20-12 overexpressing transgenic Arabidopsis thaliana lines were significantly higher, but MDA contents were lower than that of wild-type (WT) plants under heat and cold stresses, displayed enhanced thermotolerance and coldresistance. These results lay a foundation for the functional characterization of DcHSP20s and provide a candidate gene, DcHSP20-12, for improving the tolerance of D. catenatum to temperature stress in the future.

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