您好，欢迎访问广东省农业科学院 机构知识库！
广东省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Molecular Detection of Southern Tomato Amalgavirus Prevalent in Tomatoes and Its Genomic Characterization with Global Evolutionary Dynamics

文献类型： 外文期刊

作者： Hussain, Muhammad Dilshad 1 ; Farooq, Tahir 3 ; Chen, Xi 1 ; Jiang, Tong 1 ; Zang, Lianyi 5 ; Shakeel, Muhammad Taimoor 6 ; Zhou, Tao 1 ;

作者机构： 1.China Agr Univ, Dept Plant Pathol, State Key Lab Agrobiotechnol, Beijing 100193, Peoples R China

2.China Agr Univ, Dept Plant Pathol, Key Lab Pest Monitoring & Green Management, Minist Agr & Rural Affairs, Beijing 100193, Peoples R China

3.Guangdong Acad Agr Sci, Plant Protect Res Inst, Guangzhou 510640, Peoples R China

4.Guangdong Acad Agr Sci, Guangdong Prov Key Lab High Technol Plant Protect, Guangzhou 510640, Peoples R China

5.Shandong Agr Univ, Coll Plant Protect, Collaborat Innovat Ctr Fruit & Vegetable Qual & Ef, Tai An 271018, Peoples R China

6.Islamia Univ Bahawalpur, Fac Agr & Environm, Dept Plant Pathol, Bahawalpur 63100, Pakistan

关键词： southern tomato amalgavirus; cryptic pathogen; vertical transmission; viral prevalence; next-generation sequencing; phylodynamics; genetic variability

期刊名称：VIRUSES-BASEL （ 影响因子：5.818； 五年影响因子：5.811 ）

ISSN：

年卷期： 2022 年 14 卷 11 期

页码：

收录情况： SCI

摘要： Southern tomato amalgavirus (STV) is a cryptic pathogen that is abundant in tomato production fields and intensifies the resurgence of tomato yellow stunt disease (ToYSD), together with other phytoviruses. Here, we mapped the geographical and genomic diversity, phylogenetics, and evolutionary dynamics of STV. We found that STV prevailed across China and Pakistan, with a maximum average rate of infection of 43.19% in Beijing, China, and 40.08% in Punjab, Pakistan. Subsequently, we amplified, cloned, and annotated the complete genome sequences of STV isolates from Solanum lycopersicum L. in China (OP548653 and OP548652) and Pakistan (MT066231) using Sanger and next-generation sequencing (NGS). These STV isolates displayed close evolutionary relationships with others from Asia, America, and Europe. Whole-genome-based molecular diversity analysis showed that STV populations had 33 haplotypes with a gene diversity (Hd) of 0.977 and a nucleotide diversity (pi) of 0.00404. The genetic variability of RNA-dependent RNA-polymerase (RdRp) was higher than that of the putative coat protein (CP) p42. Further analysis revealed that STV isolates were likely to be recombinant but with a lower-to-moderate level of confidence. With a variable distribution pattern of positively and negatively selected sites, negative selection pressure predominantly acted on p42 and RdRp. These findings elaborated on the molecular variability and evolutionary trends among STV populations across major tomato-producing regions of the world.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广东省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台