Nuclear localization and the C-terminus region of PiAVR3b from Phytophthora infestans are required for recognition by the resistance protein Rpi-R3b
文献类型： 外文期刊
作者： Wang, Hongyang 1 ; Zhang, Yindi 1 ; Luan, Hongying 1 ; Chen, Aie 2 ; Liu, Jing 1 ; Lu, Jie 3 ; Tang, Wei 1 ; Li, Canhui 1 ;
作者机构： 1.Yunnan Normal Univ, Yunnan Key Lab Potato Biol, Kunming 650500, Yunnan, Peoples R China
2.Yunnan Normal Univ, Teaching Affairs Dept, Kunming 650500, Yunnan, Peoples R China
3.Heilongjiang Acad Agr Sci, Harbin 150086, Peoples R China
关键词： Phytophthora infestans; PiAVR3b; Effector-triggered immunity; Nuclear localization
期刊名称：EUROPEAN JOURNAL OF PLANT PATHOLOGY （ 影响因子：2.224； 五年影响因子：2.262 ）
ISSN： 0929-1873
年卷期： 2022 年 163 卷 2 期
页码：
收录情况： SCI
摘要： Recognition of avirulence (AVR) proteins by cognate plant resistance proteins leads to effector-triggered immunity. Previous reports have indicated that the Phytophthora infestans avirulence effector 3b (PiAVR3b) can trigger a hypersensitive response when recognized by resistance protein Rpi-R3b. However, the molecular mechanisms by which the PiAVR3b triggers immune responses are largely unknown. Here, we investigated the variation of PiAVR3b in diverse P. infestans isolates and found that they show the presence/absence polymorphisms. The allelic variation of PiAVR3b showed three polymorphic residues at positions 41, 85 and 124. Truncation and expression analysis showed that a 61-amino acid region in the C-terminus half of PiAVR3b was enough to trigger Rpi-R3b-mediated cell death. In addition, we found that a paralogue of PiAVR3b, PITG_18221, escaped recognition by Rpi-R3b. Microscopic imaging revealed that PiAVR3b was located in both the nucleus and cytoplasm. The nuclear localization of PiAVR3b was required for the Rpi-R3b-mediated immunity. Finally, we proved that both SGT1 and HSP90 are essential for PiAVR3b-triggered cell death. Our results provide a basis for further investigation of how PiAVR3b triggers plant immunity.
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