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Molecular cloning and expression dynamics of Atrogin-1 in muscle tissue of Russian sturgeon (Acipenser gueldenstaedtii) under high salt stress

文献类型： 外文期刊

作者： Mo, Yong-Juan 1 ; Gao, Yi-Li 2 ; Xu, Shi-Jjian 3 ; Wang, Bin 3 ; Wu, Jingping 3 ; Yu, Peng 6 ; Li, Ming 7 ; Kang, Xiangtao 7 ; Han, Zhi-Qiang 1 ;

作者机构： 1.Zhejiang Ocean Univ, Fishery Coll, Zhoushan 316022, Peoples R China

2.Ningbo Univ, Coll Sci & Technol, Ningbo Key Lab Agr Germplasm Resources Min & Envi, Cixi 315300, Peoples R China

3.Quzhou Sturgeon Aquat Food Sci & Technol Dev Co L, 1 Xunlong Rd, Quzhou 324002, Peoples R China

4.Minist Agr & Rural Affairs, Key Lab Sturgeon Genet & Breeding, Hangzhou 311799, Peoples R China

5.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China

6.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China

7.Henan Agr Univ, Coll Anim Sci & Technol, Zhengzhou 450046, Peoples R China

关键词： Atrogin-1; High salt stress; Acipenser gueldenstaedtii; Selective breeding

期刊名称：AQUACULTURE REPORTS （ 影响因子：3.7； 五年影响因子：4.0 ）

ISSN： 2352-5134

年卷期： 2025 年 40 卷

页码：

收录情况： SCI

摘要： Atrogin-1, a muscle-specific ubiquitin ligase, is integral to the regulation of protein degradation and plays a pivotal role in the targeted degradation of critical muscle signaling proteins, which can lead to skeletal muscle dysfunction. High salt stress is known to induce muscle atrophy and alter the physical and chemical properties of muscle in cultured fish. Conversely, an optimal salinity level can enhance muscle quality. The Russian sturgeon ( Acipenser gueldenstaedtii), an anadromous species of significant economic value, is potentially adaptable to brackish water environments, yet the underlying regulatory mechanisms remain elusive. To elucidate the regulatory mechanism of Atrogin-1 in response to high salt stress in Russian sturgeon, we conducted cDNA cloning and expression analysis. The cDNA encoding the Russian sturgeon protein Atrogin-1 was successfully cloned, comprising 1472 bp and encoding 353 amino acid residues (with an open reading frame of 1062 bp). Utilizing the sequence of Russian sturgeon atrogin-1, we designed novel primers and a specific anti-peptide antibody for our study, confirming that atrogin-1 is selectively expressed in skeletal muscle and heart tissues. The sturgeons were then allocated into four experimental groups for salt stress exposure; one control group was maintained in freshwater, while the others were subjected to salinities of 8 %o, 16 %o, and 24 %o, respectively. Quantitative PCR (qPCR) and western blot analysis were employed to assess Atrogin-1 expression levels under high salt stress. A significant upregulation of atrogin-1 mRNA was observed at salinities of 16 %o and 24 %o compared to the control group. Similarly, protein expression levels of Atrogin-1 were markedly elevated under the same salinity conditions. Our findings indicate that the modulation of Atrogin-1 expression is a sensitive indicator of the response to high salt stress. This research is anticipated to inform aquaculture practices and shed light on the selection of germplasm resources for the development of salt-tolerant Russian sturgeon strains.

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