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Metabolic profiling and gene expression analysis provides insights into flavonoid and anthocyanin metabolism in poplar

文献类型： 外文期刊

作者： Tian, Yuru 1 ; Li, Qianqian 1 ; Rao, Shupei 1 ; Wang, Aike 3 ; Zhang, Hechen 5 ; Wang, Liangsheng 6 ; Li, Yue 1 ; Chen, J 1 ;

作者机构： 1.Beijing Forestry Univ, Coll Biol Sci & Technol, Beijing Adv Innovat Ctr Tree Breeding Mol Design, 35 Qinghua East Rd, Beijing 100083, Peoples R China

2.Beijing Forestry Univ, Natl Engn Lab Tree Breeding, 35 Qinghua East Rd, Beijing 100083, Peoples R China

3.Yucheng Inst Agr Sci, Shangqiu 476000, Henan, Peoples R China

4.Shangqiu Zhongxing Seedling Planting Co Ltd, Shangqiu 476000, Henan, Peoples R China

5.Henan Acad Agr Sci, Hort Res Inst, Zhengzhou 450002, Henan, Peoples R China

6.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources & Beijing Bot Garden, 20 Nanxincun, Beijing 100093, Peoples R China

关键词： anthocyanin; flavonoids; gene expression; leaf coloration; metabolic profiling; poplar.

期刊名称：TREE PHYSIOLOGY （ 影响因子：4.196； 五年影响因子：4.727 ）

ISSN： 0829-318X

年卷期： 2021 年 41 卷 6 期

页码：

收录情况： SCI

摘要： Poplar, a woody perennial model, is a common and widespread tree genus. We cultivated two red leaf poplar varieties from bud mutation of Populus sp. Linn. '2025' (also known as Zhonglin 2025, L2025 for shot): Populus deltoides varieties with bright red leaves (LHY) and completely red leaves (QHY). After measuring total contents of flavonoid, anthocyanin, chlorophyll and carotenoid metabolites, a liquid chromatography-electrospray ionization-tandem mass spectrometry system was used for the relative quantification of widely targeted metabolites in leaves of three poplar varieties. A total of 210 flavonoid metabolites (89 flavones, 40 flavonols, 25 flavanones, 18 anthocyanins, 16 isoflavones, 7 dihydroflavonols, 7 chalcones, 5 proanthocyanidins and 3 other flavonoid metabolites) were identified. Compared with L2025, 48 and 8 flavonoids were more and less abundant, respectively, in LHY, whereas 51 and 9 flavonoids were more and less abundant in QHY, respectively. On the basis of a comprehensive analysis of the metabolic network, gene expression levels were analyzed by deep sequencing to screen for potential reference genes for the red leaves. Most phenylpropanoid biosynthesis pathway-involved genes were differentially expressed among the examined varieties. Gene expression analysis also revealed several potential anthocyanin biosynthesis regulators including three MYB genes. The study results provide new insights into poplar flavonoid metabolites and represent the theoretical basis for future studies on leaf coloration in this model tree species.

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