Development and characterization of 33 SNP markers in a critically endangered Hynobius amjiensis using ddRAD sequencing
文献类型： 外文期刊
作者： Kan, Xia 1 ; Wang, Guoliang 2 ; Zhang, Lili 2 ; Zhao, Xin 2 ; Shan, Shuangshuang 1 ; Liu, Guiming 2 ; Shao, Chen 1 ; Wang 1 ;
作者机构： 1.Zhejiang Normal Univ, Coll Chem & Life Sci, Jinhua 321000, Zhejiang, Peoples R China
2.Beijing Acad Agr & Forestry Sci, Beijing Agrobiotechnol Res Ctr, Beijing Key Lab Agr Genet Resources & Biotechnol, Beijing 100097, Peoples R China
关键词： Hynobius amjiensis; Single nucleotide polymorphism; Double digest restriction-site associated DNA sequencing; Genetic diversity assessment
期刊名称：CONSERVATION GENETICS RESOURCES （ 影响因子：0.973； 五年影响因子：0.946 ）
ISSN： 1877-7252
年卷期： 2021 年 13 卷 4 期
页码：
收录情况： SCI
摘要： Hynobius amjiensis was considered as one of the 29 most threatened amphibian in China. To effectively conserve, manage and recover the population of this critically endangered species, 33 single nucleotide polymorphism (SNP) markers were developed using double digest restriction-site associated DNA (ddRAD) sequencing. The minor allele frequency per locus ranged from 0.0161 to 0.5000. The observed heterozygosity and expected heterozygosity varied from 0.0323 to 0.6667 (average 0.3303) and from 0.0317 to 0.5000 (average 0.2772), respectively. The inbreeding coefficient value ranged between - 0.3315 and 0.0000. No significant deviation from Hardy-Weinberg equilibrium (P > 0.05) were found in all loci. These novel SNPs will be helpful for the population genetic assessment and conservation of H. amjiensis.
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