您好，欢迎访问中国热带农业科学院 机构知识库！
中国热带农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Antibacterial mechanism and structure-activity relationships of Bombyx mori cecropin A

文献类型： 外文期刊

作者： Tian, Yuyuan 1 ; Wei, Hongxian 4 ; Lu, Fuping 4 ; Wu, Huazhou 4 ; Lou, Dezhao 4 ; Wang, Shuchang 4 ; Geng, Tao 4 ;

作者机构： 1.Guizhou Univ, State Key Lab Green Pesticide, Guiyang, Peoples R China

2.Guizhou Univ, Key Lab Green Pesticide & Agr Bioengn, Minist Educ, Guiyang, Peoples R China

3.Guizhou Univ, Ctr R&D Fine Chem, Guiyang, Peoples R China

4.Chinese Acad Trop Agr Sci, Inst Environm & Plant Protect, Haikou, Peoples R China

关键词： antimicrobial peptide; cecropin A; cell membrane; DNA degradation; structure-activity optimization

期刊名称：INSECT MOLECULAR BIOLOGY （ 影响因子：2.3； 五年影响因子：2.8 ）

ISSN： 0962-1075

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Bombyx mori cecropin A (Bmcecropin A) has antibacterial, antiviral, anti-filamentous fungal and tumour cell inhibition activities and is considered a potential succedaneum for antibiotics. We clarified the antibacterial mechanism and structure-activity relationships and then directed the structure-activity optimization of Bmcecropin A. Firstly, we found Bmcecropin A shows a strong binding force and permeability to cell membranes like a detergent; Bmcecropin A could competitively bind to the cell membrane with the cell membrane-specific dye DiI, then damaged the membrane for the access of DiI into the cytoplasm and leading to the leakage of electrolyte and proteins. Secondly, we found Bmcopropin A could also bind to and degrade DNA; furthermore, DNA library polymerase chain reaction (PCR) results indicated that Bmcecropin A inhibited DNA replication by non-specific binding. In addition, we have identified C-terminus amidation and serine-lysine- glycine (SLG) amino acids of Bmcecropin A played critical roles in the membrane damage and DNA degradation. Based on the above results, we designed a mutant of Bmcecropin A (E-9 to H, D-17 to K, K-33 to A), which showed higher antibacterial activity, thermostability and pH stability than ampicillin but no haemolytic activity. Finally, we speculated that Bmcecropin A damaged the cell membrane through a carpet model and drew the schematic diagram of its antibacterial mechanism, based on the antibacterial mechanism and the three-dimensional configuration. These findings yield insights into the mechanism of antimicrobial peptide-pathogen interaction and beneficial for the development of new antibiotics.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国热带农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台