您好，欢迎访问中国热带农业科学院 机构知识库！
中国热带农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

A 14-amino acids deletion in BmShadow results to non-moult on the 2nd instar in the bivoltine silkworm, Bombyx mori

文献类型： 外文期刊

作者： Zhu, Juan 1 ; Chen, Yan-Rong 3 ; Geng, Tao 4 ; Tang, Shun-Ming 1 ; Zhao, Qiao-ling 1 ; Shen, Xing-Jia 1 ;

作者机构： 1.Jiangsu Univ Sci & Technol, Coll Biotechnol, Jiangsu Key Lab Sericutural Biol & Biotechnol, Zhenjiang 212018, Jiangsu, Peoples R China

2.Chinese Acad Agr Sci, Sericultural Res Inst, Key Lab Silkworm & Mulberry Genet Improvement, Minist Agr & Rural Affairs, Zhenjiang 212018, Jiangsu, Peoples R China

3.Huangshan Univ, Huangshan 245041, Peoples R China

4.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Haikou 571101, Hainan, Peoples R China

关键词： CRISPR/Cas9; Microinjection; BmShadow; 20-hydroxyecdysterone; Bombyx mori

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

ISSN： 0378-1119

年卷期： 2021 年 777 卷

页码：

收录情况： SCI

摘要： The Bombyx mori Shadow gene (BmShadow) belongs to the superfamily of cytochrome P450 genes. To elucidate the function of the BmShadow gene and its association with diapause, we employed the CRISPR/Cas9 system to knock out the BmShadow gene in the bivoltine strain Qiufeng. The mutant (BmShadow(-/-)) was obtained in G(2), exhibiting a 42-base deletion corresponded exactly to the amino acids regions from positions 155 to 168. The larvae of BmShadow(-/-) cannot moult at the pre-moulting stage of the 2nd instar. When the BmShadow(-/-) larvae were fed with 20E analogue at the late stage of the 2nd instar, they were rescued and developed into the 3rd instar. Rescue experiments indicated that the 20E concentration of BmShadow(-/-) larvae was significantly lower than that in WT larvae, and the 20E concentration of BmShadow(-/-) larvae which fed 20E analogue was restored to normal levels. Interestingly, the BmShadow(-/-) larvae could not moult on the 1st instar when they hatched from eggs after being stored at 5 degrees C for 40 days or after hibernation, suggesting that the 20E transported from the mother was partially consumed in the diapause maintenance phase. Our study confirmed that BmShadow is involved in 20E synthesis and a 14-amino acids region from position 155 to 168 was essential for its function, also there appears to be no other compensation pathway in vivo, which offered an important potential target locus for the control of silkworm development and the biological control of agricultural and forestry pests.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国热带农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台