文献类型： 外文期刊

作者： Wu, Huiliang ¹ ; Yin, Jiyuan ¹ ; Li, Siming ² ; Wang, Hao ³ ; Jiang, Peng ¹ ; Li, Pengfei ⁴ ; Ding, Zhaoyang ³ ; Yan, Han ² ; Chen, Bo ⁵ ; Wang, Linchuan ¹ ; Wang, Qing ¹ ;

作者机构： 1.South China Agr Univ, Coll Vet Med, Guangzhou 510642, Peoples R China

2.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Fishery Drug Dev,Minist Agr & Rural Affair, Guangdong Prov Key Lab Aquat Anim Immunol & Sustai, Guangzhou 510380, Peoples R China

3.Jiangxi Acad Agr Sci, Inst Qual & Safety & Stand Agr Prod Res, Nanlian Rd 602, Nanchang 330200, Peoples R China

4.Shanghai Ocean Univ, Natl Demonstrat Ctr Expt Fisheries Sci Educ, Shanghai 201306, Peoples R China

5.Guangxi Acad Sci, Guangxi Key Lab Marine Environm Sci, Nanning 530000, Peoples R China

6.Nanchang Yimen Biol Technol Co Ltd, Nanchang 330200, Peoples R China

7.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Guangzhou 510380, Peoples R China

关键词： Grass carp reovirus; VP4; Oral immunization; L. lactis; Immunogenicity; Protective efficacy

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.7； 五年影响因子：4.7 ）

ISSN： 1050-4648

年卷期： 2024 年 147 卷

页码：

收录情况： SCI

摘要： The hemorrhagic disease causing by grass carp reovirus (GCRV) infection, is associated with major economic losses and significant impact on aquaculture worldwide. VP4 of GCRV is one of the major outer capsid proteins which can induce an immune response in the host. In this study, pNZ8148-VP4/L. lactis was constructed to express recombinant VP4 protein of GCRV, which was confirmed by the Western-Blot and enzyme-linked immunosorbent assay. Then we performed the oral immunization for rare minnow model and the challenge with GCRV-II. After oral administration, pNZ8148-VP4/L. lactis can continuously reside in the intestinal tract to achieve antigen presentation. The intestinal and spleen samples were collected at different time intervals after immunization, and the expression of immune-related genes was detected by real-time fluorescence quantitative PCR. The results showed that VP4 recombinant L. lactis could induce complete cellular and humoral immune responses in the intestinal mucosal system, and effectively regulate the immunological effect of the spleen. The immunogenicity and the protective efficacy of the oral vaccine was evaluated by determining IgM levels and viral challenge to vaccinated fish, a significant level (P < 0.01) of antigen-specific IgM with GCRV-II neutralizing activity was able to be detected, which provided a effective protection in the challenge experiment. These results indicated that an oral probiotic vaccine with VP4 expression can provide effective protection for grass carp against GCRV-II challenge, suggesting a promising vaccine strategy for fish.