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3-Methyl-4-nitrophenol Exposure Deteriorates Oocyte Maturation by Inducing Spindle Instability and Mitochondrial Dysfunction

文献类型： 外文期刊

作者： Chen, Fan 1 ; Luo, An-Feng 1 ; Li, Ming-Guo 1 ; Zheng, Li-Xiang 1 ; Gu, Hao 1 ; Zhou, Chang-Fan 1 ; Zeng, Wei 1 ; Molenaar, Adrian 1 ; Ren, Hong-Yan 1 ; Bi, Yan-Zhen 1 ;

作者机构： 1.Hubei Acad Agr Sci, Inst Anim Sci & Vet Med, Key Lab Anim Embryo Engn & Mol Breeding Hubei Prov, Wuhan 430070, Peoples R China

2.Fitzherbert Res Ctr, Rumen Microbiol & Anim Nutr & Physiol AgRes, Grasslands Campus, Palmerston North 4410, New Zealand

关键词： oocyte maturation; PNMC; spindle stability; Fignl1; mitochondria

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：5.6； 五年影响因子：6.2 ）

ISSN： 1661-6596

年卷期： 2024 年 25 卷 7 期

页码：

收录情况： SCI

摘要： 3-methyl-4-nitrophenol (PNMC), a well-known constituent of diesel exhaust particles and degradation products of insecticide fenitrothion, is a widely distributed environmental contaminant. PNMC is toxic to the female reproductive system; however, how it affects meiosis progression in oocytes is unknown. In this study, in vitro maturation of mouse oocytes was applied to investigate the deleterious effects of PNMC. We found that exposure to PNMC significantly compromised oocyte maturation. PNMC disturbed the spindle stability; specifically, it decreased the spindle density and increased the spindle length. The weakened spindle pole location of microtubule-severing enzyme Fignl1 may result in a defective spindle apparatus in PNMC-exposed oocytes. PNMC exposure induced significant mitochondrial dysfunction, including mitochondria distribution, ATP production, mitochondrial membrane potential, and ROS accumulation. The mRNA levels of the mitochondria-related genes were also significantly impaired. Finally, the above-mentioned alterations triggered early apoptosis in the oocytes. In conclusion, PNMC exposure affected oocyte maturation and quality through the regulation of spindle stability and mitochondrial function.

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