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Improving the Nutritional Value and Safety of Cotton Stalk Feed via Response Surface Methodology and Co-Fermentation Techniques

文献类型: 外文期刊

作者: Li, Kunyi 1 ; Xu, Yuansheng 1 ; Guo, Kai 1 ; Cui, Weidong 1 ; Li, Yang 1 ; Hou, Min 1 ;

作者机构: 1.Xinjiang Acad Agr Sci, Inst Microbiol, Xinjiang Lab Special Environm Microbiol, Urumqi 830091, Peoples R China

2.Xinjiang Agr Univ, Coll Grassland Sci, Key Lab Western Arid Reg Grassland Resource & Ecol, Minist Educ, Urumqi 830052, Peoples R China

关键词: sustainable agriculture; Aspergillus niger; fiber degradation; gossypol

期刊名称:FERMENTATION-BASEL ( 影响因子:3.3; 五年影响因子:3.5 )

ISSN:

年卷期: 2025 年 11 卷 3 期

页码:

收录情况: SCI

摘要: Cotton stalks, a major agricultural byproduct, are challenging to decompose naturally; however, they can be transformed into valuable animal feed through microbial fermentation. This study identifies Aspergillus niger HQXY as the most efficient cellulase-producing strain out of six evaluated strains, using it to ferment cotton stalks and significantly degrade cellulose and hemicellulose. By optimizing solid-state fermentation conditions via response surface methodology, the crude fiber content of the cotton stalks was reduced by 34%. A novel sequential co-fermentation approach combining Aspergillus niger with probiotics (Bacillus licheniformis, Candida utilis, and Lactobacillus casei) further enhanced the feed's nutritional profile. The optimal results were obtained using a 1:1:1 ratio of strains (Aspergillus niger, Bacillus licheniformis, and Lactobacillus casei) at a 8% inoculation rate over 30 days. This co-fermentation strategy lowered the pH and reduced gossypol to 15.5 mgkg-1. The findings highlight the effectiveness of Aspergillus niger HQXY and probiotics in improving the quality of cotton stalks, by reducing crude fiber and gossypol content, thus offering a promising method for the sustainable utilization of agricultural waste as high-quality animal feed.

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