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A probe-free electrochemical immunosensor for methyl jasmonate based on a Cu-MOF-carboxylated graphene oxide platform

文献类型： 外文期刊

作者： Xing, Gengqi 1 ; Wang, Cheng 1 ; Liu, Ke 1 ; Luo, Bin 1 ; Hou, Peichen 1 ; Wang, Xiaodong 1 ; Dong, Hongtu 1 ; Wang, Jianshu 2 ; Li, Aixue 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Intelligent Equipment Res Ctr, Beijing 100097, Peoples R China

2.Hebei Univ Engn, Coll Landscape & Ecol Engn, Handan 056038, Peoples R China

期刊名称：RSC ADVANCES （ 影响因子：4.036； 五年影响因子：3.748 ）

ISSN：

年卷期： 2022 年 12 卷 26 期

页码：

收录情况： SCI

摘要： Methyl jasmonate (MeJA) is an important phytohormone which can regulate plant growth and stress tolerance. It is very necessary to develop sensitive and accurate detection methods for MeJA. In this work, a probe-free electrochemical immunosensor for MeJA detection was developed based on a Cu-MOF-carboxylated graphene oxide (COOH-GO) platform. The Cu2+ in the Cu-MOFs was used to provide redox signals, which avoids the application of an external redox probe in the electrolyte solutions as conventional immunosensors. COOH-GO was used to improve the structural stability and provide more sites for binding MeJA antibodies. The linear range of the MeJA immunosensor is from 10 pM to 100 mu M, which can cover the whole concentration range of MeJA in most plants. And its detection limit is very low (0.35 pM), and it can detect very low concentrations of MeJA. This immunosensor is simple, low cost, and does not need redox probe solutions for measurements. It shows remarkable potential for on-site application in precision agriculture.

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