Genome assembly of the plant pathogen Plasmodiophora brassicae reveals novel secreted proteins contributing to the infection of Brassica rapa
文献类型： 外文期刊
作者： Li, Peirong 1 ; Lv, Sirui 5 ; Zhang, Zhijun 5 ; Su, Tongbing 1 ; Wang, Weihong 1 ; Xin, Xiaoyun 1 ; Zhao, Xiuyun 1 ; Li, Xiaoman 1 ; Zhang, Deshuang 1 ; Yu, Yangjun 1 ; Ma, Tao 1 ; Liu, Guodong 7 ; Zhang, Fenglan 1 ; Yu, Shuancang 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci BAAFS, Beijing Vegetable Res Ctr BVRC, Beijing 100097, Peoples R China
2.Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr, State Key Lab Vegetable Biobreeding, Beijing 100097, Peoples R China
3.Minist Agr, Key Lab Biol & Genet Improvement Hort Crops North, Beijing 100097, Peoples R China
4.Beijing Key Lab Vegetable Germplasm Improvement, Beijing 100097, Peoples R China
5.Shandong Agr Univ, State Key Lab Crop Biol, Tai An 271018, Shandong, Peoples R China
6.Shandong Agr Univ, Coll Hort Sci & Engn, Tai An 271018, Shandong, Peoples R China
7.Agr Comprehens Serv Ctr Guide Subdist Off Changqin, Jinan 250300, Shandong, Peoples R China
关键词： Plasmodiophora brassicae; Clubroot; Brassica rapa; Genome; Secreted protein; Serine hydroxymethyltransferase
期刊名称：HORTICULTURAL PLANT JOURNAL （ 影响因子：6.2； 五年影响因子：6.1 ）
ISSN： 2095-9885
年卷期： 2025 年 11 卷 3 期
页码：
收录情况： SCI
摘要： The soil-resident pathogen, Plasmodiophora brassicae, infects cruciferous crops, causing obligate parasitic clubroot disease and posing a significant threat to the Brassica vegetable industry in China. To learn more about its pathogenesis, we reported a Nanopore sequencing-derived 25.3 Mb high-quality genome sequence of P. brassicae pathotype 4 strain (P.b 4). Comparing the P.b 4 genome with that of the published P. brassicae e3 genome (P.b e3) identified single nucleotide polymorphisms, structural variations, and small insertions and deletions. We then carried out RNA-sequencing of root samples from a clubroot-susceptible line at 5, 14, and 28 days after inoculation (DAI), and classified genes into five categories based on their expression patterns. Interestingly, 158 genes were highly expressed at 14 DAI, which were enriched in budding cell isotropic bud growth, ascospore wall assembly, spore wall assembly, spore wall biogenesis, and ascospore wall biogenesis. Subsequently, we bioinformatically predicted 555 secreted effector candidates, among which only 125 were expressed during infection and had amino acid lengths less than 400. The putative effector Pb010018, which was highly expressed at 14 DAI, was validated to have a signal peptide using a yeast secretion system. Luciferase activity and co-immunoprecipitation assays demonstrated that Pb010018 interacts with serine hydroxymethyltransferase BrSHMT1, and expression analysis showed that SHMT1 was upregulated in both Arabidopsis and B. rapa during infection. Furthermore, after infection, the Arabidopsis shmt1 mutant (atshmt1) showed reduced severity of clubroot disease, together with downregulated expression of Pb010018. Our results offer new insights into plant-pathogen interaction mechanisms, and provide the possibility for improving Brassica resistance to clubroot disease.
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