eIF2Bβ confers resistance to Turnip mosaic virus by recruiting ALKBH9B to modify viral RNA methylation
文献类型： 外文期刊
作者： Sha, Tongyun 1 ; Li, Zhangping 1 ; Xu, Shirui 1 ; Su, Tongbing 3 ; Shopan, Jannat 1 ; Jin, Xingming 1 ; Deng, Yueying 1 ; Lyu, Xiaolong 1 ; Hu, Zhongyuan 1 ; Zhang, Mingfang 1 ; Yang, Jinghua 1 ;
作者机构： 1.Zhejiang Univ, Inst Vegetable Sci, Lab Germplasm Innovat & Mol Breeding, Hangzhou, Peoples R China
2.Zhejiang Univ, Yazhou Bay Sci & Technol City, Hainan Inst, Sanya, Peoples R China
3.Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr, Beijing, Peoples R China
4.Minist Agr & Rural Affairs, Key Lab Hort Plant Growth & Dev, Hangzhou, Peoples R China
关键词： eIF2B beta; ALKBH9B; N-6-methyladenosine; natural variation; Turnip mosaic virus
期刊名称：PLANT BIOTECHNOLOGY JOURNAL （ 影响因子：10.5； 五年影响因子：12.4 ）
ISSN： 1467-7644
年卷期： 2024 年 22 卷 11 期
页码：
收录情况： SCI
摘要： Eukaryotic translation initiation factors (eIFs) are the primary targets for overcoming RNA virus resistance in plants. In a previous study, we mapped a BjeIF2B beta from Brassica juncea representing a new class of plant virus resistance genes associated with resistance to Turnip mosaic virus (TuMV). However, the mechanism underlying eIF2B beta-mediated virus resistance remains unclear. In this study, we discovered that the natural variation of BjeIF2B beta in the allopolyploid B. juncea was inherited from one of its ancestors, B. rapa. By editing of eIF2B beta, we were able to confer resistance to TuMV in B. juncea and in its sister species of B. napus. Additionally, we identified an N-6-methyladenosine (m(6)A) demethylation factor, BjALKBH9B, for interaction with BjeIF2B beta, where BjALKBH9B co-localized with both BjeIF2B beta and TuMV. Furthermore, BjeIF2B beta recruits BjALKBH9B to modify the m(6)A status of TuMV viral coat protein RNA, which lacks the ALKB homologue in its genomic RNA, thereby affecting viral infection. Our findings have applications for improving virus resistance in the Brassicaceae family through natural variation or genome editing of the eIF2B beta. Moreover, we uncovered a non-canonical translational control of viral mRNA in the host plant.
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