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Dietary Influence on Growth, Physicochemical Stability, and Antimicrobial Mechanisms of Antimicrobial Peptides in Black Soldier Fly Larvae

文献类型： 外文期刊

作者： Liu, Shaojuan 1 ; Tariq, Muhammad Raheel 1 ; Zhang, Qihui 1 ; Wang, Hui 1 ; Wang, Fei 1 ; Zheng, Chaozhong 1 ; Li, Kuntai 3 ; Zhuang, Zhikai 1 ; Wang, Leiyu 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Agr Prod Proc Res Inst, Zhanjiang 524001, Peoples R China

2.Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 524088, Peoples R China

3.Guangdong Ocean Univ, Coll Food Sci & Technol, Zhanjiang 524088, Peoples R China

4.Jiangxi Agr Univ, Coll Biol Sci & Engn, Nanchang 330045, Peoples R China

关键词： black soldier fly larvae; antimicrobial peptides; growth performance; physicochemical stability; transcriptomic analysis

期刊名称：INSECTS （ 影响因子：2.9； 五年影响因子：3.3 ）

ISSN：

年卷期： 2024 年 15 卷 11 期

页码：

收录情况： SCI

摘要： Safe antibiotic substitutes are needed given the rise in antimicrobial resistance, environmental contamination, and stringent antibiotic regulations. Insect-derived antimicrobial peptides (AMPs) are promising candidates due to their antimicrobial activity, stability, and safety. This study investigates the antimicrobial mechanism of crude AMP extracts and their physicochemical characteristics in black soldier fly larvae (BSFL). The results indicated that BSFL reared on a wheat bran diet exhibited significantly improved growth performance and AMP production when compared to the other three diets. AMP extracts showed enhanced antimicrobial activity and physicochemical stability, including temperatures and metal ions except Cu+. Moreover, AMP extracts disrupted the cell membrane and inhibited the cell cycle of Staphylococcus aureus (S. aureus), thus exhibiting antimicrobial activity. Furthermore, transcriptomic and KEGG enrichment analyses identified 509 differentially expressed genes (DEGs) related to the Toll and IMD signaling pathways. STRING and GeneMANIA analyses confirmed the association of these pathways with immune response and AMP secretion. qRT-PCR results showed elevated expression of immune genes (GNBP3, NFKBIA, GADD45, and Spz) in BSFL following S. aureus immunization, consistent with RNA-seq findings. These findings offer a valuable reference for using AMPs as antibiotic substitutes in animal feeds and highlight the need for further research on AMP purification and the synergistic regulation of protein synthesis and AMP production in BSFL.

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